TotalSeq™-C0932 anti-human Lymphotoxin &beta-上海雅吉生物科技有限公司

规格	价格	库存
10µg	￥ 4428	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human LT-βR Fc protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA

Preparation

The antibody was purified by chromatography and conjugated with TotalSeq?-C oligomer under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

PG - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq?-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling?Solution.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 ?g per million cells in 100 ?L volume. Refer to the corresponding TotalSeq? protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq? products. For example, for any technology platform Buyer uses with TotalSeq?, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq? with that platform.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting¹, immunoprecipitation¹, ELISA detection¹, induction of apoptosis². The biotinylated 31G4D8 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with the purified 36G9G3 antibody (Cat. No. 322101/322102) as capture antibody. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 322003/322004).

Additional Product Notes

TotalSeq? reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g.?10x Genomics Chromium System and Reagents) and sequencer (e.g Illumina analyzers) are required. Please contact?technical support?for more information, or visit?biolegend.com/totalseq.

The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Scientific Poster Library.

Application References (PubMed link indicates BioLegend citation)

Developer's communication
Chen M-C, et al. 2003. J. Biol. Chem. 278:16073.

RRID

AB_2910390 (BioLegend Cat. No. 322013)

Antigen Details

Structure

Member of the TNF receptor superfamily; transmembrane receptor containing 3 TNF receptor domains, predicted molecular weight approximately 47 kD

Distribution

Highly expressed in lung, liver, and kidney. Moderate expression in heart and testes; weak expression in brain, thymus, spleen, and lymph nodes.

Function

Promotes apoptosis via TRAF3 and TRAF5, plays a role in the development of lymphoid organs

Interaction

Receptor for heterotrimeric lymphotoxin complex (composed of LTα and LTβ as well as LIGHT. Also interacts with TRAF3, TRAF4, and TRAF5 as well as hepatitis C virus core protein

Cell Type

Embryonic Stem Cells

Biology Area

Immunology, Stem Cells

Molecular Family

Cytokine/Chemokine Receptors

Antigen References