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Purified anti-mouse CD90.2 (Thy1.2) (Maxpar® R
产品名称:
Purified anti-mouse CD90.2 (Thy1.2) (Maxpar® R
产品类别:
抗体
产品编号:
105333
产品应用:
105333
[价格]
规格 价格 库存
100µg ¥ 2196 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF? - Verified

Recommended Usage

This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.

Application Notes

Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion1,2,7, costimulation of CD3/TCR-mediated signal transduction3,4, and immunohistochemical staining5 of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301 & 101302) is recommended prior to immunofluorescent staining. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 105351 & 105352).

Additional Product Notes

Maxpar? is a registered trademark of Standard BioTools Inc.

Application References

(PubMed link indicates BioLegend citation)
  1. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  2. Seaman WE. 1983. J. Immunol. 130:1713. (Deplete)
  3. Nakashima I, et al. 1991. J. Immunol. 147:1153. (Costim)
  4. Nakashima I, et al. 1993. J. Immunol. 151:3511. (Costim)
  5. Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (IHC)
  6. Hardy B, et al. 2005. Int. Immunol. 17:615.
  7. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  8. Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
  9. Sungur CM, et al. 2013. PNAS. 110:7401. PubMed
Product Citations
  1. Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed
  2. Wang Y, et al. 2021. Cancer Cell. :. PubMed
  3. McClellan BL, et al. 2022. STAR Protoc. 3:101357. PubMed
RRID
AB_2563765 (BioLegend Cat. No. 105333)

Antigen Details

Structure
Ig superfamily, 25-35 kD
Distribution

Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice

Function
Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor
CD45
Cell Type
Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Craig W, et al. 1993. J. Exp. Med. 177:1331.
3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1.
4. Mayani H, et al. 1994. Blood 83:2410.

Gene ID
21838 View all products for this Gene ID
UniProt
View information about CD90.2 on UniProt.org

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