PE/Fire™ 810 anti-mouse CD90.2 (Thy1.2) Anti-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1524	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse thymus or spleen

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Fire? 810 under optimal conditions.

Concentration

0.2 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤?0.06 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire? 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm.

Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire? 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion^1,2,7, costimulation of CD3/TCR-mediated signal transduction^3,4, and immunohistochemical staining⁵ of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301 & 101302) is recommended prior to immunofluorescent staining. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 105351 & 105352).

Application References (PubMed link indicates BioLegend citation)

Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
Seaman WE. 1983. J. Immunol. 130:1713. (Deplete)
Nakashima I, et al. 1991. J. Immunol. 147:1153. (Costim)
Nakashima I, et al. 1993. J. Immunol. 151:3511. (Costim)
Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (IHC)
Hardy B, et al. 2005. Int. Immunol. 17:615.
Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
Sungur CM, et al. 2013. PNAS. 110:7401. PubMed

RRID

AB_2927836 (BioLegend Cat. No. 105357)

Antigen Details

Structure: Ig superfamily, 25-35 kD
Distribution: Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice
Function: Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor: CD45
Cell Type: Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Craig W, et al. 1993. J. Exp. Med. 177:1331.
3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1.
4. Mayani H, et al. 1994. Blood 83:2410.
Gene ID: 21838 View all products for this Gene ID
UniProt: View information about CD90.2 on UniProt.org

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