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首页 > 抗体蛋白 > 抗体
FITC anti-mouse CD90.2 (Thy1.2) Antibody
产品名称：
FITC anti-mouse CD90.2 (Thy1.2) Antibody
产品类别：
抗体
产品编号：
105305
产品应用：
105305
[价格]
规格 价格 库存
50µg ￥ 648 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion1,2,7, costimulation of CD3/TCR-mediated signal transduction3,4, and immunohistochemical staining5 of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301 & 101302) is recommended prior to immunofluorescent staining. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 105351 & 105352).

Application References

(PubMed link indicates BioLegend citation)
  1. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  2. Seaman WE. 1983. J. Immunol. 130:1713. (Deplete)
  3. Nakashima I, et al. 1991. J. Immunol. 147:1153. (Costim)
  4. Nakashima I, et al. 1993. J. Immunol. 151:3511. (Costim)
  5. Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (IHC)
  6. Hardy B, et al. 2005. Int. Immunol. 17:615.
  7. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  8. Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
  9. Sungur CM, et al. 2013. PNAS. 110:7401. PubMed
Product Citations
  1. Yang AC, et al. 2020. Nature. 583:425. PubMed
  2. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  3. He Y, et al. 2021. Infect Immun. 89:. PubMed
  4. Wang D, et al. 2018. Immunity. 48:659. PubMed
  5. Silva C, et al. 2019. Cell Physiol Biochem. 52:503. PubMed
  6. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  7. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  8. Chimenti I, et al. 2022. Cell Death Discov. 8:149. PubMed
  9. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  10. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  11. Pereira JL, et al. 2021. Transl Oncol. 14:101125. PubMed
  12. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  13. Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed
  14. Van Braeckel-Budimir N, et al. 2018. Cell Rep. 24:3374. PubMed
  15. Westbrook A, et al. 2012. Mutagenesis. 27:77. PubMed
  16. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  17. Zhang Y, et al. 2022. Vaccines (Basel). 10:. PubMed
  18. Yang P et al. 2019. Cell Syst. 8(5):427-445 . PubMed
  19. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  20. Ni T, et al. 2016. Nat Cell Biol. 18:1221-1232. PubMed
  21. Baja?a S, et al. 2022. iScience. 25:103732. PubMed
  22. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  23. Sugii S, et al. 2010. Proc Natl Acad Sci U S A. 107:3558. PubMed
  24. Kaji T, et al. 2016. Int Immunol. 28: 267 - 282. PubMed
  25. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  26. Tanaka S, et al. 2021. Am J Transplant. 21:353. PubMed
  27. Tanaka K, et al. 2009. Cell Stem Cell. 4:217. PubMed
  28. Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
  29. Hu J, et al. 2022. Stem Cell Res Ther. 13:349. PubMed
  30. Barwick BG, et al. 2018. Nat Commun. 9:1900. PubMed
  31. Fearon AE, et al. 2021. iScience. 24:103143. PubMed
RRID
AB_313176 (BioLegend Cat. No. 105305) AB_313177 (BioLegend Cat. No. 105306)

Antigen Details

Structure
Ig superfamily, 25-35 kD
Distribution

Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice

Function
Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor
CD45
Cell Type
Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Craig W, et al. 1993. J. Exp. Med. 177:1331.
3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1.
4. Mayani H, et al. 1994. Blood 83:2410.

Gene ID
21838 View all products for this Gene ID
UniProt
View information about CD90.2 on UniProt.org

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