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Alexa Fluor® 700 anti-mouse CD90.2 (Thy1.2) An
产品名称：
Alexa Fluor® 700 anti-mouse CD90.2 (Thy1.2) An
产品类别：
抗体
产品编号：
105319
产品应用：
105319
[价格]
规格 价格 库存
25µg ￥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.?The suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion1,2,7, costimulation of CD3/TCR-mediated signal transduction3,4, and immunohistochemical staining5 of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301 & 101302) is recommended prior to immunofluorescent staining. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 105351 & 105352).

Application References

(PubMed link indicates BioLegend citation)
  1. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  2. Seaman WE. 1983. J. Immunol. 130:1713. (Deplete)
  3. Nakashima I, et al. 1991. J. Immunol. 147:1153. (Costim)
  4. Nakashima I, et al. 1993. J. Immunol. 151:3511. (Costim)
  5. Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (IHC)
  6. Hardy B, et al. 2005. Int. Immunol. 17:615.
  7. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  8. Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
  9. Sungur CM, et al. 2013. PNAS. 110:7401. PubMed
Product Citations
  1. Kwong Chung CK, et al. 2017. Immunology. 150:221. PubMed
  2. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  3. Krausgruber T, et al. 2020. Nature. 583:296. PubMed
  4. Herz J, et al. 2015. J Exp Med. 212: 1153 - 1169. PubMed
  5. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  6. AR P, et al. 2016. Circ Res. 118: 400-409. PubMed
  7. Emg?rd J, et al. 2018. Immunity. 143:419. PubMed
  8. Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
  9. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
  10. Watanabe M, et al. 2020. Nat Commun. 4.808333333. PubMed
  11. Lu P, et al. 2021. Nat Commun. 12:1042. PubMed
  12. Seedhom M, et al. 2012. J Virol. 86:6010. PubMed
  13. Schuldt N, et al. 2015. PLoS One. 10: 0145762. PubMed
  14. Monticelli L, et al. 2015. Proc Natl Acad Sci U S A. 112: 10762-10767. PubMed
  15. Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
  16. Rive CM, et al. 2022. Mol Ther Methods Clin Dev. 26:4. PubMed
  17. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  18. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  19. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
  20. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  21. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
  22. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  23. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  24. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  25. Goc J, et al. 2021. Cell. :. PubMed
RRID
AB_493724 (BioLegend Cat. No. 105319) AB_493725 (BioLegend Cat. No. 105320)

Antigen Details

Structure
Ig superfamily, 25-35 kD
Distribution

Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice

Function
Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor
CD45
Cell Type
Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Craig W, et al. 1993. J. Exp. Med. 177:1331.
3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1.
4. Mayani H, et al. 1994. Blood 83:2410.

Gene ID
21838 View all products for this Gene ID
UniProt
View information about CD90.2 on UniProt.org

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