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Alexa Fluor® 488 anti-mouse CD90.2 (Thy1.2) An
产品名称：
Alexa Fluor® 488 anti-mouse CD90.2 (Thy1.2) An
产品类别：
抗体
产品编号：
105315
产品应用：
105315
[价格]
规格 价格 库存
25µg ￥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume.?For immunohistochemical staining on frozen tissue sections, a concentration range of 5.0 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion1,2,7, costimulation of CD3/TCR-mediated signal transduction3,4, and immunohistochemical staining5 of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301 & 101302) is recommended prior to immunofluorescent staining. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 105351 & 105352).

Application References

(PubMed link indicates BioLegend citation)
  1. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  2. Seaman WE. 1983. J. Immunol. 130:1713. (Deplete)
  3. Nakashima I, et al. 1991. J. Immunol. 147:1153. (Costim)
  4. Nakashima I, et al. 1993. J. Immunol. 151:3511. (Costim)
  5. Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (IHC)
  6. Hardy B, et al. 2005. Int. Immunol. 17:615.
  7. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  8. Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
  9. Sungur CM, et al. 2013. PNAS. 110:7401. PubMed
Product Citations
  1. Todd E, et al. 2011. J Immunol. 187:3015. PubMed
  2. Wu N, et al. 2020. Cell Rep. 30:1129. PubMed
  3. Amano C, et al. 2015. PLoS One. 10: 0137207. PubMed
  4. Kretschmer S, et al. 2013. PLoS One. 8:e55201. PubMed
  5. Wang Y, et al. 2021. Nat Commun. 12:2343. PubMed
  6. Armaka M, et al. 2022. Genome Med. 14:78. PubMed
  7. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  8. García-Bonilla M, et al. 2020. Stem Cell Res Ther. 11:121. PubMed
  9. Schliehe C, et al. 2012. J Virol. 139:3442. PubMed
  10. Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed
  11. Wang Y, et al. 2022. Front Immunol. 13:847581. PubMed
  12. Armaka M, et al. 2018. Nat Commun. 9:618. PubMed
  13. Mohammed RN, et al. 2019. Sci Rep. 4.185416667. PubMed
  14. Prasai A, et al. 2017. Stem Cell Rev.. 10.1007/s12015-017-9721-9. PubMed
RRID
AB_492887 (BioLegend Cat. No. 105315) AB_492886 (BioLegend Cat. No. 105316)

Antigen Details

Structure
Ig superfamily, 25-35 kD
Distribution

Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice

Function
Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor
CD45
Cell Type
Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Craig W, et al. 1993. J. Exp. Med. 177:1331.
3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1.
4. Mayani H, et al. 1994. Blood 83:2410.

Gene ID
21838 View all products for this Gene ID
UniProt
View information about CD90.2 on UniProt.org

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