PE/Fire™ 700 anti-mouse CD45 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1692	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse thymus or spleen

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Fire? 700 under optimal conditions.

Concentration

0.2 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC -?Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire? 700 has a maximum excitation of 565 nm and a maximum emission of 695 nm.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation³, complement-dependent cytotoxicity^1,5,?immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)^4,6, Western blotting⁷, and spatial biology (IBEX)^10,11. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References (PubMed link indicates BioLegend citation)

Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
Liu F, et al. 2012. Blood. 119:3295. PubMed
Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

RRID

AB_2876405 (BioLegend Cat. No. 103177) AB_2876405 (BioLegend Cat. No. 103178)

Antigen Details

Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: All hematopoietic cells except mature erythrocytes and platelets
Function: Phosphatase, T and B cell activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type: B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 19264 View all products for this Gene ID
UniProt: View information about CD45 on UniProt.org

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