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首页 > 抗体蛋白 > 抗体
PE/Cyanine5 anti-mouse CD45 Antibody
产品名称：
PE/Cyanine5 anti-mouse CD45 Antibody
产品类别：
抗体
产品编号：
103109
产品应用：
103109
[价格]
规格 价格 库存
25µg ￥ 984 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5,?immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  11. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Weng L, et al. 2016. Sci Rep. 6:38632. PubMed
  2. Thelin MA, et al. 2017. Diabetes. 66:2220. PubMed
  3. Fujimori S, et al. 2022. Elife. 11:. PubMed
  4. Zhang Y, et al. 2018. Sci Rep. 8:5994. PubMed
  5. Cieslik KA, et al. 2018. J Gerontol A Biol Sci Med Sci. 73:1167. PubMed
  6. Ohigashi I, et al. 2021. Cell Rep. 36:109657. PubMed
  7. Jones RE et al. 2019. Cell Rep. 28(11):2757-2766 . PubMed
  8. Moose DL, et al. 2020. Cell Rep. 30:3864. PubMed
  9. Ohigashi I, et al. 2020. Cell Reports. 29(9):2901-2916.e6.. PubMed
  10. Nguyen SL, et al. 2021. Sci Rep. 11:4217. PubMed
  11. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  12. Vazquez S, et al. 2015. Exp Hematol. 43: 578-585. PubMed
  13. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  14. Gulati GS, et al. 2018. Nat Protoc. 13:1294. PubMed
  15. Kumar B, et al. 2020. Haematologica. Online ahead of print:. PubMed
  16. Chen I, et al. 2016. Sci Rep. 6:27195. PubMed
  17. Lasso P, et al. 2020. Front Immunol. 584959:11. PubMed
  18. Han D, et al. 2021. J Immunol Res. 6688053:2021. PubMed
  19. Sibilio A, et al. 2022. iScience. 25:103790. PubMed
  20. Li X, et al. 2020. Aging Dis. 0.821527778. PubMed
  21. Sun L, et al. 2013. J Immunol. 191:3210. PubMed
  22. Kwok T, et al. 2022. Front Aging. 3:838943. PubMed
  23. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  24. Ni Y, et al. 2021. Theranostics. 11:9431. PubMed
  25. Menon S, et al. 2021. Nat Commun. 12:4640. PubMed
  26. Zhou H, et al. 2016. J Immunol. 197: 288 - 295. PubMed
  27. Sen A, et al. 2012. Proc Natl Acad Sci U S A. 109:20667. PubMed
  28. Bansal K, et al. 2017. Nat Immunol. 18:263-273. PubMed
  29. Lerbs T, et al. 2020. JCI Insight. 5:00. PubMed
RRID
AB_312974 (BioLegend Cat. No. 103109) AB_312975 (BioLegend Cat. No. 103110)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

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