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Brilliant Violet 785™ anti-mouse CD45 Antibo
产品名称：
Brilliant Violet 785™ anti-mouse CD45 Antibo
产品类别：
抗体
产品编号：
103149
产品应用：
103149
[价格]
规格 价格 库存
50µg ￥ 3276 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785? under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 785? excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5,?immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  11. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  2. Sun L, et al. 2021. Cancer Cell. :. PubMed
  3. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  4. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  5. Lee H, et al. 2020. Cell Metabolism. 31(4):822-836. PubMed
  6. Myers JA, et al. 2022. JCI Insight. :. PubMed
  7. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  8. Rogic A, et al. 2021. Nat Commun. 12:6889. PubMed
  9. Ford MJ, et al. 2021. Cell Rep. 36:109677. PubMed
  10. Demircioglu F, et al. 2020. Nat Commun. 11:1290. PubMed
  11. Kuang Z, et al. 2020. Antib Ther. 3:227. PubMed
  12. Gómez-Díaz C, et al. 2021. iScience. 24:103241. PubMed
  13. Ostendorf BN, et al. 2020. Nat Med. 26:1048. PubMed
  14. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  15. Kienzl M, et al. 2020. Oncoimmunology. 9:1776059. PubMed
  16. Privratsky JR, et al. 2018. Am J Physiol Renal Physiol. 315:F682. PubMed
  17. Burns JC, et al. 2021. Bio Protoc. 11:e4091. PubMed
  18. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  19. Yin Q, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed
  20. Johnson LA, et al. 2021. Life Sci Alliance. :4. PubMed
  21. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  22. Pereira JA, et al. 2020. Hum Mol Genet. 29:1253. PubMed
  23. Tavazoie MF, et al. 2018. Cell. 172:825. PubMed
  24. Burns JC, et al. 2020. eLife. 9:00. PubMed
  25. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  26. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
  27. Qadir AS, et al. 2021. iScience. 24:103348. PubMed
  28. Chen F, et al. 2022. Cell Rep. 38:110215. PubMed
  29. Lu X, et al. 2019. Circ Res. 125:1055. PubMed
  30. Maniati E, et al. 2020. Cell Rep. 30:525. PubMed
  31. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  32. Baja?a S, et al. 2022. iScience. 25:103732. PubMed
  33. Santopaolo M, et al. 2021. Front Immunol. 609406:12. PubMed
  34. Lang JF et al. 2019. Nat Commun. 10(1):3415 . PubMed
  35. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  36. Freed-Pastor WA, et al. 2021. Cancer Cell. :. PubMed
  37. Chen B, et al. 2021. Cell. 184:6262. PubMed
  38. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  39. Lee HN, et al. 2022. JCI Insight. 7:. PubMed
  40. Krisna SS, et al. 2021. STAR Protoc. 2:100627. PubMed
  41. Filtjens J, et al. 2021. Nat Commun. 12:2936. PubMed
  42. Deets KA, et al. 2021. Elife. 10:. PubMed
  43. Fearon AE, et al. 2021. iScience. 24:103143. PubMed
RRID
AB_2564590 (BioLegend Cat. No. 103149)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

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