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Brilliant Violet 711™ anti-mouse CD45 Antibo
产品名称：
Brilliant Violet 711™ anti-mouse CD45 Antibo
产品类别：
抗体
产品编号：
103147
产品应用：
103147
[价格]
规格 价格 库存
50µg ￥ 3144 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711? under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 711? excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5,?immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  11. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  2. Qi Z, et al. 2022. Nat Commun. 13:182. PubMed
  3. Harel M, et al. 2020. Cell. 179(1):236-250.e18.. PubMed
  4. Burrack AL, et al. 2019. Cell Rep. 28:2140. PubMed
  5. Fitzgerald AA, et al. 2021. J Immunother Cancer. 9:. PubMed
  6. Park JA, et al. 2021. J Hematol Oncol. 14:142. PubMed
  7. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
  8. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  9. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
  10. Hole CR, et al. 2019. Nat Commun. 10:2955. PubMed
  11. Sodji QH, et al. 2022. Cancer Res Commun. 2:725. PubMed
  12. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
  13. Stump CT, et al. 2021. Open Biol. 11:210245. PubMed
  14. Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed
  15. Xu S, et al. 2021. Immunity. . PubMed
  16. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  17. Bachofner M, et al. 2017. Dev Cell. 42:616. PubMed
  18. Lewis EL, et al. 2021. Front Immunol. 12:741518. PubMed
  19. Cardenas JJ, et al. 2020. Methods Enzymol. 415:631. PubMed
  20. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  21. Zhang SL, et al. 2022. Gut Microbes. 14:2046246. PubMed
  22. Lin J, et al. 2021. Nat Commun. 12:5321. PubMed
  23. Beura LK, et al. 2018. Immunity. 48:327. PubMed
  24. Robles-Oteiza C, et al. 2021. Dis Model Mech. 14:. PubMed
  25. Wu S, et al. 2021. Nat Cancer. 2:189. PubMed
  26. Johann K, et al. 2021. Nat Commun. 12:5981. PubMed
  27. Ivan DC, et al. 2021. Front Immunol. 12:666961. PubMed
  28. Silva DA, et al. 2019. Nature. 565:186. PubMed
  29. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  30. Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed
  31. Ryu S, et al. 2021. eLife. 0.416666666666667. PubMed
  32. Tao Z, et al. 2022. Cells. 11:. PubMed
  33. Olivera GC, et al. 2021. Elife. 10:. PubMed
  34. Lin J, et al. 2021. Cancer Immunol Res. 9:1413. PubMed
  35. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  36. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  37. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  38. McGinley AM, et al. 2020. Immunity. 52(2):342-356. PubMed
  39. Quijano-Rubio A, et al. 2022. Nat Biotechnol. Online ahead of print. PubMed
RRID
AB_2564383 (BioLegend Cat. No. 103147)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

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