热线电话: 021-34661275
产品分类/PRODUCT CLASSIFICATION

上海细胞库

人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|

原代细胞

原代细胞| 永生化细胞|

细胞培养

血清| 无血清冻存| 细胞培养试剂盒| 普通培养基| 完全培养基| 细胞污染预防| 细胞污染清除| 细胞培养辅助试剂|

细胞服务

STR鉴定服务| ATCC代购服务|

酶联试剂盒

人ELISA试剂盒| 大鼠ELISA试剂盒| 小鼠ELISA试剂盒| 兔ELISA试剂盒| 猪ELISA试剂盒| 鱼ELISA试剂盒| 牛ELISA试剂盒| 犬ELISA试剂盒| 鸭ELISA试剂盒| 豚鼠ELISA试剂盒| 鸡ELISA试剂盒| 绵羊ELISA试剂盒| 山羊ELISA试剂盒| 仓鼠ELISA试剂盒| 植物ELISA试剂盒| 药残留类试剂盒| 其他ELISA试剂盒|

生化试剂盒

生化试剂盒| 微量法检测盒| 分光法检测盒| 胶体金检测盒|

PCR试剂盒

荧光探针PCR试剂盒| 染料荧光PCR试剂盒| 核酸扩增/纯化| 普通PCR检测试剂盒| 质粒菌种感受态|

抗体蛋白

蛋白多肽| 抗体| 免疫组化| 免疫球蛋白|

试剂耗材

标准试液| 科研试剂| 缓 冲 液| 实验耗材| 高效液相色谱对照品|

首页 > 抗体蛋白 > 抗体
Biotin anti-mouse CD45 Antibody
产品名称：
Biotin anti-mouse CD45 Antibody
产品类别：
抗体
产品编号：
103103
产品应用：
103103
[价格]
规格 价格 库存
50µg ￥ 648 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5,?immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  11. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Yen W, et al. 2012. Clin Cancer Res. 18:5374. PubMed
  2. Dell'Aringa M, et al. 2018. Mucosal Immunol. 1.207638889. PubMed
  3. Tarban N, et al. 2022. Cells. 11:. PubMed
  4. Fujimori S, et al. 2022. Elife. 11:. PubMed
  5. Lv X, et al. 2022. Acta Pharm Sin B. 12:735. PubMed
  6. Kaminitz A, et al. 2013. Int Immunol. 25:485. PubMed
  7. Larsen SB, et al. 2021. Cell Stem Cell. :. PubMed
  8. Chiang HC, et al. 2018. Sci Rep. 8:2731. PubMed
  9. Xie T, et al. 2021. Sci Adv. 7: . PubMed
  10. Muhsin-Sharafaldine M, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.03.138. PubMed
  11. Wagner F, et al. 2016. J Bone Joint Surg Am. 98: 916 - 925. PubMed
  12. Nair S, et al. 2016. Nat Commun. 7:10913. PubMed
  13. Fu R, et al. 2019. Nat Commun. 10:3210. PubMed
  14. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  15. Kurane T, et al. 2020. Okajimas Folia Anat Jpn. 96:49:00. PubMed
  16. Ibrahim AM, et al. 2021. Breast Cancer Res. 23:90. PubMed
  17. Karaman S, et al. 2022. J Exp Med. 219:. PubMed
  18. Sitaraman S, et al. 2021. JCI Insight. 6:. PubMed
  19. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  20. Howard E, et al. 2020. J Allergy Clin Immunol. . PubMed
  21. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  22. Nevius E, et al. 2015. J Exp Med. 212: 1931 - 1946. PubMed
  23. Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
  24. Wang Z, et al. 2022. Cell Mol Immunol. 19:210. PubMed
  25. Shen Z, et al. 2021. iScience. 24:103014. PubMed
  26. Lv J, et al. 2021. Cell Discov. 7:24. PubMed
  27. Chatterjee S, et al. 2014. PLoS One. 9:87858. PubMed
  28. Ludwik KA, et al. 2021. STAR Protocols. 2(1):100270. PubMed
  29. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  30. Adam RC et al. 2018. Cell stem cell. 22(3):398-413 . PubMed
  31. Watson JK, et al. 2020. Sci Rep. 10:10490. PubMed
  32. Zhu H, et al. 2020. J Bone Miner Res. 35:698. PubMed
  33. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
  34. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  35. Sitaraman S, et al. 2019. Sci Rep. 9:12509. PubMed
  36. Lü?nd F, et al. 2022. STAR Protoc. 3:101438. PubMed
  37. Pilling D, Gomer R 2014. PLoS One. 9:93730. PubMed
  38. Pual VM, et al. 2019. PLOS Biology. 17(10):e3000492. PubMed
  39. Barnes SE, et al. 2021. J Neuroinflammation. 18:199. PubMed
  40. Sountoulidis A, et al. 2012. PLoS One. 7:e41460. PubMed
  41. Kwiecien K, et al. 2020. Sci Rep. 10:13702. PubMed
  42. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  43. Buffolo M, et al. 2019. Cell Rep. 29:270. PubMed
  44. Fu NY, et al. 2018. Dev Cell. 47:629. PubMed
  45. Chen Z, et al. 2020. Cell Death Dis. 11:180. PubMed
  46. Budai Z, et al. 2021. Cells. 10:. PubMed
  47. De Micheli AJ, et al. 2020. Cell Rep. 30:3583. PubMed
  48. Aoto M, et al. 2019. FEBS Open Bio. 9:291. PubMed
  49. E O’Koren, R Mathew, D Saban 2016. Sci Rep. 6:20636. PubMed
  50. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  51. Seong J, et al. 2018. Development. 145:14. PubMed
  52. Samuelson DR, et al. 2021. Commun Biol. 4:997. PubMed
RRID
AB_312968 (BioLegend Cat. No. 103103) AB_312969 (BioLegend Cat. No. 103104)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

联系我们

TEL：021-34661275 　点击拨打热线