热线电话: 021-34661275
产品分类/PRODUCT CLASSIFICATION

上海细胞库

人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|

原代细胞

原代细胞| 永生化细胞|

细胞培养

血清| 无血清冻存| 细胞培养试剂盒| 普通培养基| 完全培养基| 细胞污染预防| 细胞污染清除| 细胞培养辅助试剂|

细胞服务

STR鉴定服务| ATCC代购服务|

酶联试剂盒

人ELISA试剂盒| 大鼠ELISA试剂盒| 小鼠ELISA试剂盒| 兔ELISA试剂盒| 猪ELISA试剂盒| 鱼ELISA试剂盒| 牛ELISA试剂盒| 犬ELISA试剂盒| 鸭ELISA试剂盒| 豚鼠ELISA试剂盒| 鸡ELISA试剂盒| 绵羊ELISA试剂盒| 山羊ELISA试剂盒| 仓鼠ELISA试剂盒| 植物ELISA试剂盒| 药残留类试剂盒| 其他ELISA试剂盒|

生化试剂盒

生化试剂盒| 微量法检测盒| 分光法检测盒| 胶体金检测盒|

PCR试剂盒

荧光探针PCR试剂盒| 染料荧光PCR试剂盒| 核酸扩增/纯化| 普通PCR检测试剂盒| 质粒菌种感受态|

抗体蛋白

蛋白多肽| 抗体| 免疫组化| 免疫球蛋白|

试剂耗材

标准试液| 科研试剂| 缓 冲 液| 实验耗材| 高效液相色谱对照品|

首页 > 抗体蛋白 > 抗体
Alexa Fluor® 700 anti-mouse CD45 Antibody
产品名称：
Alexa Fluor® 700 anti-mouse CD45 Antibody
产品类别：
抗体
产品编号：
103127
产品应用：
103127
[价格]
规格 价格 库存
25µg ￥ 1128 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5,?immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  11. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Stoupa A, et al. 2018. EMBO Mol Med. 10:. PubMed
  2. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  3. Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
  4. Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed
  5. Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed
  6. Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed
  7. Ren Z, et al. 2021. EMBO Molecular Medicine. :e14059. PubMed
  8. Trittel S, et al. 2019. Sci Rep. 9:16362. PubMed
  9. Fu R, et al. 2020. Sci Rep. 10:1455. PubMed
  10. Guzzi N et al. 2018. Cell. 173(5):1204-1216 . PubMed
  11. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  12. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  13. Daskou M, et al. 2022. PLoS Pathog. 18:e1010160. PubMed
  14. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  15. Zhang R, et al. 2021. Cell Mol Immunol. 18:1222. PubMed
  16. Xi H, et al. 2016. J Exp Med. 213: 189 - 207. PubMed
  17. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  18. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  19. Gimblet C et al. 2017. Cell host & microbe. 22(1):13-24 . PubMed
  20. Putnam NE, et al. 2019. PLoS Pathog. 15:e1007744. PubMed
  21. Chen J, et al. 2021. Sci Adv. 7:. PubMed
  22. Ghosh S, et al. 2022. APL Bioeng. 6:036105. PubMed
  23. Abou-Hamad J, et al. 2022. iScience. 25:105524. PubMed
  24. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  25. Katsura A, et al. 2017. Mol Oncol. 11:1241. PubMed
  26. Amir M, et al. 2018. Cell Rep. 25:3733. PubMed
  27. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  28. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  29. Zhang C, et al. 2022. J Extracell Vesicles. 11:e12209. PubMed
  30. Ma C, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
  31. Fujii Y, et al. 2022. JBMR Plus. 6:e10562. PubMed
  32. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
  33. Evren E, et al. 2020. Immunity. 54(2):259-275.e7. PubMed
  34. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  35. Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed
  36. Egusquiza RJ, et al. 2020. Environ Health Perspect. 128:47011. PubMed
  37. McDowell SAC, et al. 2021. Nat Cancer. 2:545. PubMed
  38. Reyes RM, et al. 2021. Oncoimmunology. 10:2006529. PubMed
  39. Lebratti T, et al. 2021. eLife. 10:00. PubMed
  40. Vackova J, et al. 2020. Int J Mol Sci. 21:00. PubMed
  41. Yang R, et al. 2012. J Immunol. 189:2656. PubMed
  42. Kienzl M, et al. 2020. Oncoimmunology. 9:1776059. PubMed
  43. Cao W, et al. 2017. Immunity. 47:1182. PubMed
  44. van Elsas MJ, et al. 2022. Int J Mol Sci. 23:. PubMed
  45. Stutchfield B, et al. 2015. Gastroenterology. 149: 1896-1909.e14. PubMed
  46. Doty DT, et al. 2020. Int J Mol Sci. 21:00. PubMed
  47. Sun L, et al. 2020. J Immunol. 2177:204. PubMed
  48. Magupalli VG, et al. 2020. Science. :369. PubMed
  49. Li J, et al. 2020. Cancer Discov. . PubMed
  50. Daubeuf F, et al. 2017. Curr Protoc Mouse Biol. 10.1002/cpmo.26. PubMed
  51. Emg?rd J, et al. 2018. Immunity. 143:419. PubMed
  52. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  53. Koliaraki V et al. 2019. Cell reports. 26(3):536-545 . PubMed
  54. Sophie Thiemann et al. 2017. Cell host & microbe. 21(6):682-694 . PubMed
  55. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  56. Tanaka S, et al. 2012. J Immunol. 188:6145. PubMed
  57. Wei JL, et al. 2021. J Immunother Cancer. 9: . PubMed
  58. Fantauzzi MF, et al. 2021. ERJ Open Res. 7: . PubMed
  59. Daubeuf F, et al. 2021. Cells. 10:. PubMed
  60. An J, et al. 2022. iScience. 25:103570. PubMed
  61. Chen ELY, et al. 2021. Cell Rep. 35:109227. PubMed
  62. Menzel L, et al. 2022. STAR Protoc. 3:101267. PubMed
  63. Abu El Maaty MA, et al. 2022. Sci Adv. 8:eabo2295. PubMed
  64. Carozza JA, et al. 2020. Nat Cancer. 184:1. PubMed
  65. Levi J, et al. 2020. J Nucl Med. . PubMed
  66. Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed
  67. Li J, et al. 2020. Cancer Immunol Res. 0.529166667. PubMed
  68. Duque-Correa MA, et al. 2022. Nat Commun. 13:1725. PubMed
  69. Mu?oz NM, et al. 2022. Sci Rep. 12:14449. PubMed
  70. He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed
  71. Tomlinson KL, et al. 2021. Nat Commun. 12:1399. PubMed
  72. Benne N, et al. 2020. Adv Healthc Mater. 9:e2000043. PubMed
  73. Prados A, et al. 2021. Nat Immunol. 22:510. PubMed
  74. Coursey T, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.83. PubMed
  75. Bogie JF, et al. 2020. Ther Adv Chronic Dis. 11:2040622320947378. PubMed
  76. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
  77. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  78. Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed
  79. Hossain DMS, et al. 2018. J Clin Invest. 128:644. PubMed
  80. Grzelak A, et al. 2018. Int J Mol Sci. 19:. PubMed
  81. Guérin MV, et al. 2019. Nat Commun. 10:4131. PubMed
  82. Li J, et al. 2018. Immunity. 49:178. PubMed
  83. Garcia LR, et al. 2021. Nat Commun. 12:3364. PubMed
  84. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  85. Zhao F, et al. 2022. Nat Commun. 13:6117. PubMed
  86. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  87. Abboud D, et al. 2015. Sci Rep. 5: 14746. PubMed
  88. O'Connor T, et al. 2020. Cancer Cell. 36(3):250-267. PubMed
  89. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  90. Acker KP, et al. 2019. iScience. 19:281. PubMed
  91. Schmid MC, et al. 2022. Nat Commun. 13:1768. PubMed
  92. Chen X, et al. 2022. Front Immunol. 13:828319. PubMed
  93. He X, et al. 2022. Cancer Immunol Res. 10:314. PubMed
  94. Xiong H, et al. 2021. Theranostics. 11:1594. PubMed
  95. Frank E, et al. 2016. Toxicol Pathol. 10.1177/0192623315620587. PubMed
  96. Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
  97. Sitaraman S, et al. 2019. Sci Rep. 9:12509. PubMed
  98. Shannon JP, et al. 2021. STAR Protoc. 2:100790. PubMed
  99. Park HB, et al. 2020. Nat Microbiol. 1319:5. PubMed
  100. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  101. Kostadinova E, et al. 2016. Sci Rep. 6:30943. PubMed
  102. Leyva‐Castillo JM et al. 2019. Immunity. 50(5):1262-1275 . PubMed
  103. Avraham S, et al. 2019. Oncogene. 38:3812. PubMed
  104. Uderhardt S, et al. 2019. Cell. 177:541. PubMed
  105. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  106. Zhang HG, et al. 2022. Cell Res. :. PubMed
  107. Zhai K, et al. 2021. Nat Cancer. 2:1136. PubMed
  108. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  109. Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
  110. Clark JT, et al. 2021. eLife. 10:00. PubMed
  111. Nanou A, et al. 2021. Cell Reports. 35(8):109168. PubMed
  112. Ahn D, et al. 2021. Cell Reports. 35(9):109196. PubMed
  113. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  114. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  115. Crauste F, et al. 2017. Cell Syst. 0.379166667. PubMed
  116. Nair S, et al. 2021. JCI Insight. 6:. PubMed
  117. Ma J, et al. 2021. J Transl Med. 19:477. PubMed
  118. Artham S, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L750:318. PubMed
  119. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  120. Ji G, et al. 2019. Bone Joint J. 101-B:108. PubMed
  121. Spangenberg E, et al. 2019. Nat Commun. 10:3758. PubMed
  122. Stevenson ER, et al. 2022. J Pharmacol Exp Ther. 382:356. PubMed
  123. Murray MP, et al. 2022. Cell Rep. 38:110209. PubMed
  124. Dustin CM, et al. 2021. J Immunol. 206:2989. PubMed
  125. Pizzurro GA, et al. 2021. Cancers (Basel). 13:. PubMed
  126. Bollenbach M, et al. 2021. Molecules. 26:. PubMed
  127. Grajchen E, et al. 2020. J Neuroinflammation. 0.863888889. PubMed
  128. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  129. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  130. Zhang MS, et al. 2022. Nat Commun. 13:954. PubMed
  131. Gicheva N, et al. 2016. Biochem Biophys Res Commun. 479:1-4. PubMed
  132. Natale CA, et al. 2018. Elife. 7. PubMed
  133. Ayturk UM, et al. 2020. J Bone Miner Res. 35:1981. PubMed
  134. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  135. He X, et al. 2021. J Immunother Cancer. 9:. PubMed
  136. Xiao Y, et al. 2021. Cell. 184:6037. PubMed
  137. Cambridge E, et al. 2017. Exp Hematol. 45:64-68.e5. PubMed
  138. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  139. Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
  140. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  141. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  142. Korin B, et al. 2020. Sleep. :43. PubMed
  143. Van der Meer JM, et al. 2020. Cancer Immunol Immunother. . PubMed
  144. Aguilar-Pimentel A, et al. 2017. PLoS One. 12(6):e0178563. PubMed
  145. Bommareddy PK, et al. 2019. J Biol Methods. 6:2. PubMed
  146. Hayashida E, et al. 2019. J Neuroinflammation. 0.789583333. PubMed
  147. Schaftenaar FH, et al. 2019. Sci Rep. 9:17391. PubMed
  148. Molgora M, et al. 2020. Cell. 182:886. PubMed
  149. Zhang Z, et al. 2020. Front Immunol. 11:583276. PubMed
  150. Jiang Y, et al. 2021. Nat Commun. 12:742. PubMed
  151. Melhem NJ, et al. 2021. Circulation. 143:566. PubMed
  152. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  153. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  154. Maulhardt HA, et al. 2020. Invest New Drugs. 1618:38. PubMed
  155. Wang M, et al. 2021. iScience. 24(7):102766. PubMed
  156. Guey B, et al. 2014. Proc Natl Acad Sci U S A. 111:17254. PubMed
  157. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  158. Hartwig T et al. 2017. Molecular cell. 65(4):730-742 . PubMed
  159. Litwinoff EMS, et al. 2017. Obes Res Clin Pract. 12:174. PubMed
  160. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
  161. Dye BR, et al. 2020. Biomaterials. 234:119757. PubMed
  162. Lee GR, et al. 2021. JCI Insight. 6:. PubMed
  163. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  164. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
  165. Tian F, et al. 2016. Nat Commun. 7:13283. PubMed
  166. Sen D, et al. 2016. PLoS One. 11:e0165064. PubMed
  167. Peltzer N, et al. 2018. Nature. 557:112. PubMed
  168. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  169. O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed
  170. Pflügler S, et al. 2020. Commun Biol. 3:252. PubMed
  171. Gangoso E, et al. 2021. Cell. 184:2454. PubMed
  172. Pattabiraman G, et al. 2021. Am J Physiol Renal Physiol. . PubMed
  173. Strait AA, et al. 2021. Commun Biol. 4:1005. PubMed
  174. Trivedi S, et al. 2020. Elife. 9:00. PubMed
  175. Laczkó D, et al. 2020. Immunity. 53:724. PubMed
  176. Harvey RE et al. 2017. Endocrinology. 158(7):2179-2189 . PubMed
  177. Wolf Y, et al. 2019. Cell. 179:219. PubMed
  178. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
  179. Rossi G, et al. 2022. Sci Rep. 12:13380. PubMed
  180. Hohsfield LA, et al. 2021. Elife. 10:. PubMed
RRID
AB_493714 (BioLegend Cat. No. 103127) AB_493715 (BioLegend Cat. No. 103128)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

联系我们

TEL：021-34661275 　点击拨打热线