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Alexa Fluor® 647 anti-mouse CD45 Antibody
产品名称：
Alexa Fluor® 647 anti-mouse CD45 Antibody
产品类别：
抗体
产品编号：
103123
产品应用：
103123
[价格]
规格 价格 库存
25µg ￥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC, 3D IHC - Verified
IHC, SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for other applications.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5,?immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  11. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Osterloh A, et al. 2016. Infect Immun . . PubMed
  2. Moderzynski K, et al. 2016. PLoS Negl Trop Dis. . PubMed
  3. Yan Y, et al. 2021. Immunity. 54:499. PubMed
  4. Cho J, et al. 2016. Nat Commun. 7:13373. PubMed
  5. Donato C, et al. 2020. Cell Reports. 32(10):108105. PubMed
  6. Boal-Carvalho I, et al. 2020. EMBO Rep. 21:e50421. PubMed
  7. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  8. Moore SM, et al. 2019. Transl Psychiatry. 9:24. PubMed
  9. Biffi G, et al. 2018. Cancer Discov. 2:282. PubMed
  10. Hu K, et al. 2015. PLoS One. 10: 0137123. PubMed
  11. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  12. Sutton NR, et al. 2019. Arterioscler Thromb Vasc Biol. 40:61. PubMed
  13. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  14. Fang EF, et al. 2019. Nat Neurosci. 22:401. PubMed
  15. Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed
  16. Piedrafita G, et al. 2020. Nat Commun. 11:1429. PubMed
  17. Donato C, et al. 2021. STAR Protocols. 2(2):100480. PubMed
  18. Pineda CM, et al. 2019. J Cell Biol. 218:3212. PubMed
  19. Kang MH, et al. 2020. Nat Commun. 3.186805556. PubMed
  20. Levesque S, et al. 2019. Oncoimmunology. 8:e1657375. PubMed
  21. Kuhn JA, et al. 2021. Elife. 10:. PubMed
  22. Sim?es FC, et al. 2020. Nat Commun. 0.875. PubMed
  23. Tummers B, et al. 2020. Immunity. 52(6):994-1006.e8. PubMed
  24. Huang L, et al. 2018. Exp Neurol. 300:41. PubMed
  25. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  26. Wang S, et al. 2021. Commun Biol. 22:. PubMed
  27. Shibuya M, et al. 2021. iScience. 24:103131. PubMed
  28. de Winde CM, et al. 2021. J Cell Sci. 134: . PubMed
  29. Ovadya Y, et al. 2018. Nat Commun. 9:5435. PubMed
  30. Yomtoubian S, et al. 2020. Cell Reports. 30(3):755-770.e6.. PubMed
  31. Lu YJ, et al. 2021. Cell Rep. 36:109696. PubMed
  32. Thacker VV, et al. 2020. Elife. 9:00. PubMed
  33. Sanders K, et al. 2015. Cancer Immunol Res. 3: 891-901. PubMed
  34. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  35. Bakalar MH et al. 2018. Cell. 174(1):131-142 . PubMed
  36. Chen W, et al. 2016. Nat Commun. 7: 11302. PubMed
  37. Xiong J et al. 2018. Molecular cell. 69(4):689-698 . PubMed
  38. Williams IM, et al. 2018. J Clin Invest. 128:699. PubMed
  39. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  40. Oni TE, et al. 2020. J Exp Med. :217. PubMed
  41. Prabakaran T, et al. 2021. EBioMedicine. 66:103314. PubMed
RRID
AB_493534 (BioLegend Cat. No. 103123) AB_493533 (BioLegend Cat. No. 103124)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

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