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首页 > 抗体蛋白 > 抗体
Alexa Fluor® 488 anti-mouse CD45 Antibody
产品名称：
Alexa Fluor® 488 anti-mouse CD45 Antibody
产品类别：
抗体
产品编号：
103122
产品应用：
103122
[价格]
规格 价格 库存
100µg ￥ 2748 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5,?immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  11. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
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  5. Kazakevych J, et al. 2020. Genome Biol. 21:64. PubMed
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  7. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
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  12. Nagai Y, et al. 2019. Front Immunol. 10:174. PubMed
  13. Martínez-Sabadell A, et al. 2022. STAR Protoc. 3:101712. PubMed
  14. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  15. Bornes L, et al. 2021. Life Sci Alliance. 4:00. PubMed
  16. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  17. DeDreu J, et al. 2020. FASEB J. 34:9316. PubMed
  18. Dos Santos Dias L, et al. 2021. PLoS Pathog. e1009324:17. PubMed
  19. Dubey LK, et al. 2019. Cell Rep. 27:2442. PubMed
  20. Yip HYK, et al. 2021. STAR Protocols. 2(3):100765. PubMed
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  23. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
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  27. Scarneo SA, et al. 2022. Sci Rep. 12:18091. PubMed
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  29. Burns JC, et al. 2020. eLife. 9:00. PubMed
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  32. Wu H, et al. 2021. Cell Death Discov. 7:225. PubMed
  33. Yip HYK, et al. 2020. Molecular Cell. 80(2):279-295.e8. PubMed
  34. Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed
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RRID
AB_493532 (BioLegend Cat. No. 103121) AB_493531 (BioLegend Cat. No. 103122)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

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