热线电话: 021-34661275
产品分类/PRODUCT CLASSIFICATION

上海细胞库

人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|

原代细胞

原代细胞| 永生化细胞|

细胞培养

血清| 无血清冻存| 细胞培养试剂盒| 普通培养基| 完全培养基| 细胞污染预防| 细胞污染清除| 细胞培养辅助试剂|

细胞服务

STR鉴定服务| ATCC代购服务|

酶联试剂盒

人ELISA试剂盒| 大鼠ELISA试剂盒| 小鼠ELISA试剂盒| 兔ELISA试剂盒| 猪ELISA试剂盒| 鱼ELISA试剂盒| 牛ELISA试剂盒| 犬ELISA试剂盒| 鸭ELISA试剂盒| 豚鼠ELISA试剂盒| 鸡ELISA试剂盒| 绵羊ELISA试剂盒| 山羊ELISA试剂盒| 仓鼠ELISA试剂盒| 植物ELISA试剂盒| 药残留类试剂盒| 其他ELISA试剂盒|

生化试剂盒

生化试剂盒| 微量法检测盒| 分光法检测盒| 胶体金检测盒|

PCR试剂盒

荧光探针PCR试剂盒| 染料荧光PCR试剂盒| 核酸扩增/纯化| 普通PCR检测试剂盒| 质粒菌种感受态|

抗体蛋白

蛋白多肽| 抗体| 免疫组化| 免疫球蛋白|

试剂耗材

标准试液| 科研试剂| 缓 冲 液| 实验耗材| 高效液相色谱对照品|

首页 > 抗体蛋白 > 抗体
Alexa Fluor® 488 anti-mouse CD45 Antibody
产品名称：
Alexa Fluor® 488 anti-mouse CD45 Antibody
产品类别：
抗体
产品编号：
103121
产品应用：
103121
[价格]
规格 价格 库存
25µg ￥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5,?immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  11. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Preda MB, et al. 2021. Cell Death Dis. 12:566. PubMed
  2. Kuo PC, et al. 2021. Brain Commun. 3:fcab187. PubMed
  3. Marko C, et al. 2014. Invest Ophthalmol Vis Sci . 55:291. PubMed
  4. Giorgetti E, et al. 2020. Cell Reports. 29(6):1539-1554.e7.. PubMed
  5. Kazakevych J, et al. 2020. Genome Biol. 21:64. PubMed
  6. Sakai M, et al. 2020. Immunity. 51(4):655-670. PubMed
  7. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  8. Logan C, Bowen C, and Menko A 2017. Sci Rep. . 10.1038/s41598-017-16456-5. PubMed
  9. Khandelwal P, et al. 2013. PLoS One. 8:64193. PubMed
  10. Masschelein E, et al. 2020. Skelet Muscle. 10:21. PubMed
  11. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  12. Nagai Y, et al. 2019. Front Immunol. 10:174. PubMed
  13. Martínez-Sabadell A, et al. 2022. STAR Protoc. 3:101712. PubMed
  14. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  15. Bornes L, et al. 2021. Life Sci Alliance. 4:00. PubMed
  16. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  17. DeDreu J, et al. 2020. FASEB J. 34:9316. PubMed
  18. Dos Santos Dias L, et al. 2021. PLoS Pathog. e1009324:17. PubMed
  19. Dubey LK, et al. 2019. Cell Rep. 27:2442. PubMed
  20. Yip HYK, et al. 2021. STAR Protocols. 2(3):100765. PubMed
  21. Chen I, et al. 2016. Sci Rep. 6:27195. PubMed
  22. Hallett JM, et al. 2022. Cell Stem Cell. 29:355. PubMed
  23. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
  24. Duarte J, et al. 2016. J Immunol. 197(12):4838-4847. PubMed
  25. Jung Y, et al. 2021. Nat Commun. 12:3872. PubMed
  26. Gutknecht M, et al. 2017. Nat Commun. 10.1038/s41467-017-00488-6. PubMed
  27. Scarneo SA, et al. 2022. Sci Rep. 12:18091. PubMed
  28. Mizbani A, et al. 2016. Development. 143: 4137 - 4148. PubMed
  29. Burns JC, et al. 2020. eLife. 9:00. PubMed
  30. Ferrari G, et al. 2011. Invest Ophthalmol Vis Sci. 52:2532. PubMed
  31. Wara AK, et al. 2020. Cell Rep. 33:108550. PubMed
  32. Wu H, et al. 2021. Cell Death Discov. 7:225. PubMed
  33. Yip HYK, et al. 2020. Molecular Cell. 80(2):279-295.e8. PubMed
  34. Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed
  35. Dobson HE, et al. 2020. Mucosal Immunol. 0.901388889. PubMed
  36. Biton M et al. 2018. Cell. 175(5):1307-1320 . PubMed
  37. Lustgarten Guahmich N, et al. 2020. Angiogenesis. 23:443. PubMed
  38. Limbad C, et al. 2022. iScience. 25:103848. PubMed
  39. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  40. Shibad V, et al. 2021. Front Immunol. 12:722451. PubMed
  41. Wüthrich M, et al. 2021. MBio. 12:e0201821. PubMed
  42. Li D, et al. 2022. Commun Biol. 5:271. PubMed
  43. Braun D, et al. 2013. J Biol Chem. 288:2689. PubMed
  44. Pang M, et al. 2009. J Immunol. 182:7001. PubMed
  45. Ding H, et al. 2016. Nat Commun. 7:11533. PubMed
  46. Wu X, et al. 2018. Cell. 172:423. PubMed
  47. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
  48. Franklin DA, et al. 2020. JCI Insight. 5:. PubMed
  49. Kazakevych J, et al. 2019. Sci Rep. 9:10410. PubMed
  50. Steele NG, et al. 2021. Clin Cancer Res. 27:2023. PubMed
  51. Kuo P, et al. 2016. J Am Heart Assoc. 5: 002610. PubMed
  52. Markworth JF, et al. 2020. JCI Insight. 5:00. PubMed
  53. Reed-Geaghan EG, et al. 2020. J Exp Med. 217:00:00. PubMed
  54. Peng W, et al. 2018. Mol Cell Biol. 38:e00427. PubMed
  55. Pantelidou C, et al. 2022. NPJ Breast Cancer. 8:102. PubMed
  56. Lasch M, et al. 2020. Front Physiol. 11:576736. PubMed
  57. Li Q, et al. 2020. Am J Pathol. 190:2453. PubMed
  58. RL M, et al. 2015. Proc Natl Acad Sci U S A. 112:6506-6514. PubMed
  59. Tian F, et al. 2016. Nat Commun. 7:13283. PubMed
  60. Lee EKS, et al. 2018. Cell Host Microbe. 23:121. PubMed
  61. Wang Y, et al. 2019. Front Cell Infect Microbiol. 9:286. PubMed
  62. Ye Y, et al. 2011. Am J Physiol Lung Cell Mol Physiol. 300:L216. PubMed
  63. Yang FM, et al. 2022. Front Immunol. 13:918241. PubMed
  64. Xiang W, et al. 2020. Signal Transduct Target Ther. 0.374305556. PubMed
  65. Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed
  66. Qi S, et al. 2020. Theranostics. 10:1814. PubMed
  67. Lal JC, et al. 2021. Breast Cancer Res. 23:83. PubMed
RRID
AB_493532 (BioLegend Cat. No. 103121) AB_493531 (BioLegend Cat. No. 103122)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

联系我们

TEL：021-34661275 　点击拨打热线