Ultra-LEAF™ Purified anti-human CD11c Antibo-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2844	1

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon, Chimpanzee, Squirrel Monkey

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
CyTOF? - Verified
IHC, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood.? It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 3.9 preferentially binds the activated form of CD11c, is specific for the I domain of CD11c, and is able to partially block the binding of CD11c and ICAM-4. 3.9 binding is divalent cation dependent¹². While analyzing blood, it is best to use heparin as the anti-coagulant and not EDTA. Since the ability of clone 3.9 to bind to its target is divalent cation dependent, the usage of EDTA as an anti-coagulant may be detrimental to staining due to its chelating properties.

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁴, and functional assays^5,6. The LEAF? purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301616). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 301632) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Application References (PubMed link indicates BioLegend citation)

Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York.
McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
Vainer B, et al. 2000. Am. J. Surg. Pathol. 24:1115. (IHC)
Ottonello L, et al. 1999. Blood 93:3505.
Metelitsa LS, et al. 2002. Blood 99:4166.
Sadhu C, et al. 2007. J. Leukoc. Biol. doi:10.1189/jlb.1106680. PubMed
Ihanus E, et al. 2007. Blood 109:802-810.
Gurer C, et al. 2008. Blood 112:1231. PubMed
Asai A, et al. 2009. J. Lipid Res. 50:95. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Sadhu C, et al. 2008. J. Immunoass. Immunoch. 29:42. (FC)

RRID

AB_2814120 (BioLegend Cat. No. 301631) AB_2561480 (BioLegend Cat. No. 301632)

Antigen Details

Structure: Integrin, type I transmembrane glycoprotein, associates with integrin β₂ (CD18), 145-150 kD
Distribution: Myeloid, dendritic cells, NK cells, B cells and T cell subsets
Function: Adhesion, CTL killing
Ligand/Receptor: CD54, fibrinogen, iC3b, ICAM-1, ICAM-4
Cell Type: B cells, Dendritic cells, Neutrophils, NK cells, T cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Petty H. 1996. Immunol. Today 17:209.
2. Springer T. 1994. Cell 76:301.
3. Ihanus E, et al. 2007. Blood 109:802-810.
Gene ID: 3687 View all products for this Gene ID
UniProt: View information about CD11c on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们