Purified anti-human CD20 Antibody
- 产品名称：
- Purified anti-human CD20 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 302301
- 产品应用：
- 302301
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human tonsillar B cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF?, IHC-F - Verified
IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 ?g per million cells in 100 ?l volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation4 and immunohistochemical staining of acetone-fixed frozen sections5.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
- Polyak MJ, et al. 2002. Blood 99:3256. (IP)
- Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
- Product Citations
-
- Tornack J, et al. 2017. PLoS One. 12:e0169119. PubMed
- Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed
- Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
- Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed
- Ercolano G, et al. 2021. Nat Commun. 12:2538. PubMed
- Gruber CN, et al. 2020. Immunity. 53(3):672-684. PubMed
- Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
- Gunawan M, et al. 2017. Sci Rep. . 10.1038/s41598-017-16999-7. PubMed
- Kong R, et al. 2019. Cell. 178:567. PubMed
- Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed
- Cottineau J, et al. 2017. J Clin Invest. 127:1991. PubMed
- Olin A, et al. 2018. Cell. 174:1277. PubMed
- Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed
- Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
- Ehinger E, et al. 2021. Cardiovasc Res. 117:1166. PubMed
- Henrick BM, et al. 2021. Cell. . PubMed
- Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
- Lee YS, et al. 2021. J Immunother Cancer. 9:. PubMed
- Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
- Manes TL, et al. 2020. Free Radic Biol Med. 147:102. PubMed
- Saber MM, et al. 2022. Antibodies (Basel). 11:. PubMed
- Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed
- Murayama M, et al. 2015. Nat Commun. 6: 8483. PubMed
- Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
- Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
- Joas S, et al. 2020. Cell Rep. 30:2261. PubMed
- Ravell JC, et al. 2020. J Clin Invest. 130:507. PubMed
- Ainai A, et al. 2020. Microbiol Immunol. 64:313. PubMed
- Li Z, et al. 2020. J Clin Lab Anal. 34:e23155. PubMed
- RRID
- AB_314249 (BioLegend Cat. No. 302301) AB_314250 (BioLegend Cat. No. 302302)
- Structure
- Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
- Distribution
-
B cell, T cell subsets
- Function
- B cell activation
- Ligand/Receptor
- Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
- Cell Type
- B cells, T cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Hultin L, et al. 1993. Cytometry 14:196.
2. Tedder T, et al. 1994. Immunol. Today 15:450.
- Gene ID
- 931 View all products for this Gene ID
- UniProt
- View information about CD20 on UniProt.org
