FITC anti-human CD20 Antibody
- 产品名称：
- FITC anti-human CD20 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 302303
- 产品应用：
- 302303
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human tonsillar B cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation4 and immunohistochemical staining of acetone-fixed frozen sections5.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
- Polyak MJ, et al. 2002. Blood 99:3256. (IP)
- Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
- Product Citations
-
- Qiu J, et al. 2020. Cell Rep. 33:108465. PubMed
- Rosa TLSA, et al. 2022. Front Med (Lausanne). 9:899998. PubMed
- Serra–Peinado C, et al. 2019. Nat Commun. 10:3705. PubMed
- Hagan T, et al. 2020. Cell. 178(6):1313-1328.e13.. PubMed
- Hasaart KAL, et al. 2020. Sci Rep. 10:12991. PubMed
- Laustsen A, et al. 2021. Elife. 10:. PubMed
- Panwar B, et al. 2021. Genome Res. 31:659. PubMed
- Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed
- Cao Y, et al. 2022. Nature. :. PubMed
- Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
- Appios A, et al. 2021. Bio Protoc. 11:e4044. PubMed
- Cao Y, et al. 2022. Nature. 602:657. PubMed
- Lewis SA, et al. 2021. Front Immunol. 12:724015. PubMed
- Izmirly AM, et al. 2022. PLoS Pathog. 18:e1009903. PubMed
- Song TZ, et al. 2021. Front Endocrinol (Lausanne). 12:745984. PubMed
- Sureshchandra S, et al. 2022. Cell Rep. 39:110938. PubMed
- Janela B, et al. 2019. Immunity. 50:1069. PubMed
- Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed
- Lu J, et al. 2021. Front Immunol. 12:732612. PubMed
- Paidi SK, et al. 2021. Biosens Bioelectron. 190:113403. PubMed
- Louka E, et al. 2021. Journal of Experimental Medicine. 218(2):. PubMed
- Rodriguez-Meira A, et al. 2020. STAR Protoc. 1:100125. PubMed
- Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
- Wong GK, et al. 2019. JCI Insight. 4. PubMed
- Bradley D, et al. 2022. Nat Commun. 13:5606. PubMed
- Saber MM, et al. 2022. Antibodies (Basel). 11:. PubMed
- Hu Q, et al. 2021. Nat Commun. 12:2186. PubMed
- Danzer H, et al. 2020. Elife. 9:00. PubMed
- Buters TP, et al. 2021. Br J Clin Pharmacol. Online ahead of print. PubMed
- Foote JR, et al. 2019. Front Immunol. 10:188. PubMed
- Beyer AI, et al. 2017. Stem Cells Dev. 26:102. PubMed
- Vanoni G, et al. 2021. eLife. 10:00. PubMed
- Martin GE, et al. 2018. Front Immunol. 1.019444444. PubMed
- Rodriguez‐Meira A et al. 2019. Molecular cell. 73(6):1292-1305 . PubMed
- RRID
- AB_314251 (BioLegend Cat. No. 302303) AB_314252 (BioLegend Cat. No. 302304)
- Structure
- Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
- Distribution
-
B cell, T cell subsets
- Function
- B cell activation
- Ligand/Receptor
- Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
- Cell Type
- B cells, T cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Hultin L, et al. 1993. Cytometry 14:196.
2. Tedder T, et al. 1994. Immunol. Today 15:450.
- Gene ID
- 931 View all products for this Gene ID
- UniProt
- View information about CD20 on UniProt.org
