Brilliant Violet 570™ anti-human CD20 Antibo
- 产品名称：
- Brilliant Violet 570™ anti-human CD20 Antibo
- 产品类别：
- 抗体
- 产品编号：
- 302331
- 产品应用：
- 302331
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2580 |
1 |
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human tonsillar B cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 570? under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
Brilliant Violet 570? excites at 405 nm and emits at 570 nm. The bandpass filter 585/42 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 570? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation4 and immunohistochemical staining of acetone-fixed frozen sections5.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
- Polyak MJ, et al. 2002. Blood 99:3256. (IP)
- Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
- Product Citations
-
- Townsley SM, et al. 2021. Cell Host Microbe. 29(4):564-578.e9. PubMed
- Nganou-Makamdop K, et al. 2021. Cell. 184(15):3899-3914.e16. PubMed
- Pauthner MG, et al. 2019. Immunity. 50:241. PubMed
- Gagne M, et al. 2022. Cell. 185:113. PubMed
- Harrington WE, et al. 2021. Cell Reports Medicine. 2(4):100253. PubMed
- Shangguan S, et al. 2021. Elife. 10:. PubMed
- Manuzak J, et al. 2016. J Immunol. 196: 2401 - 2409. PubMed
- Smedley J, et al. 2016. PLoS One. 11: 0157535. PubMed
- Glaros V, et al. 2021. Immunity. 54:2005. PubMed
- Amodio D, et al. 2018. J Immunol. 200:538. PubMed
- Raabe V, et al. 2020. Front Microbiol. 11:561530. PubMed
- Townsley SM, et al. 2021. STAR Protocols. 2(3):100771. PubMed
- Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed
- Stone D, et al. 2021. Hum Gene Ther. 32:96. PubMed
- Darrah PA, et al. 2019. NPJ Vaccines. 4:21. PubMed
- Fisher BS, et al. 2020. PLoS One. 15:e0233577. PubMed
- Moysi E, et al. 2021. Front Immunol. 12:683396. PubMed
- RRID
- AB_10895757 (BioLegend Cat. No. 302331) AB_2563805 (BioLegend Cat. No. 302332)
- Structure
- Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
- Distribution
-
B cell, T cell subsets
- Function
- B cell activation
- Ligand/Receptor
- Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
- Cell Type
- B cells, T cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Hultin L, et al. 1993. Cytometry 14:196.
2. Tedder T, et al. 1994. Immunol. Today 15:450.
- Gene ID
- 931 View all products for this Gene ID
- UniProt
- View information about CD20 on UniProt.org
