Alexa Fluor® 700 anti-human CD20 Antibody
- 产品名称：
- Alexa Fluor® 700 anti-human CD20 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 302322
- 产品应用：
- 302322
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human tonsillar B cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation4 and immunohistochemical staining of acetone-fixed frozen sections5.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
- Polyak MJ, et al. 2002. Blood 99:3256. (IP)
- Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
- Product Citations
-
- Basu M, et al. 2020. Cell Rep Med. :1. PubMed
- Schmitz T, et al. 2021. J Reprod Immunol. 148:103424. PubMed
- Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
- Wang LT, et al. 2020. Immunity. 53:733. PubMed
- , et al. 2021. Eur J Immunol. 51:2708. PubMed
- Sefik E, et al. 2021. Nat Biotechnol. . PubMed
- Seydoux E, et al. 2021. Cell Reports. 35(5):109084. PubMed
- Weinfurter J, et al. 2011. PLoS One. 6:e1002381. PubMed
- Wang C, et al. 2020. Oncologist. 25:382. PubMed
- Pandey M, et al. 2021. Clin Transl Immunology. 10:e1260. PubMed
- Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed
- Harris M, et al. 2013. J Virol. 87:9353. PubMed
- Kerkman P, et al. 2016. Ann Rheum Dis. 75: 1170 - 1176. PubMed
- Piepenbrink M, et al. 2016. PLoS One. 11: 0158641. PubMed
- Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
- RRID
- AB_493753 (BioLegend Cat. No. 302322)
- Structure
- Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
- Distribution
-
B cell, T cell subsets
- Function
- B cell activation
- Ligand/Receptor
- Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
- Cell Type
- B cells, T cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Hultin L, et al. 1993. Cytometry 14:196.
2. Tedder T, et al. 1994. Immunol. Today 15:450.
- Gene ID
- 931 View all products for this Gene ID
- UniProt
- View information about CD20 on UniProt.org
