Ultra-LEAF™ Purified anti-mouse/rat/human MC-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1320	1

Product Details

Verified Reactivity

Mouse, Rat, Human

Reported Reactivity

Rhesus

Antibody Type

Monoclonal

Host Species

Hamster

Immunogen

CHO-expressed, recombinant mouse MCP-1

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

ELISA Capture - Quality tested
ELISPOT Capture, Neut, WB, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 5.0 - 10 ?g/mL is recommended. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 15 pg/mL are recommended for each ELISA plate.

Application Notes

ELISA or ELISPOT Capture¹: The Ultra-LEAF? 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. 506002) as the detecting antibody for the detection of mouse MCP-1. The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody (Cat. No. 502609) as the detecting antibody for the detection of human MCP-1.
Flow Cytometry²: The fluorochrome-labeled 2H5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP-1 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization^1,4,6: The Ultra-LEAF? purified antibody (Cat. Nos. 505911 & 505912) is recommended for neutralization.
Additional reported applications (for the relevant formats) include: Western blotting³, immunohistochemistry of paraformaldehyde-fixed, saponin-treated frozen tissue sections⁵, and acetone-fixed frozen tissue sections⁷.

Application References (PubMed link indicates BioLegend citation)

Luo, Y., et al. 1994. J. Immunol. 153:3708.
Zhang, Y., et al. 2002. J. Immunol. 168:3088.
Luo, Y., et al. 1999 J. Immunol. 163:3985.
Morrison, B. E., et al. 2003 J. Clin. Invest. 112:1862.
Hancock, W. W., et al. 1997 Transplantation 64:696.
Yu, R., et al. 2006 Obesity 14:1353.
Taal MW, et al. 2001. Am. J. Physiol. Renal. Physiol. 280:F343.
Wan W, et al. 2015. Cardiovasc Res. 106:478. PubMed

Product Citations

Feng H, et al. 2020. Cell Death Dis. 1.094444444. PubMed

RRID

AB_2820086 (BioLegend Cat. No. 505911) AB_2820087 (BioLegend Cat. No. 505912) AB_2820088 (BioLegend Cat. No. 505913) AB_2820089 (BioLegend Cat. No. 505914) AB_2820090 (BioLegend Cat. No. 505915) AB_2820091 (BioLegend Cat. No. 505916)

Antigen Details

Structure: CC chemokine; dimer; 13.8 kD (Mammalian)
Bioactivity: Chemoattractant; regulates adhesion molecule expression, cytokine production in monocytes; proliferation/activation of CC-chemokine activated killer cells
Cell Sources: Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells
Cell Targets: Monocytes, basophils, T cells, NK cells, immature dendritic cells
Receptors: CCR2; CCR5; CCR10; D6; possibly L-CCR
Biology Area: Cell Biology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Bischoff, S., et al. 1992. J. Exp. Med. 175:1271.
3. Charo, I., et al. 1994. P. Natl. Acad. Sci. USA 91:2752.
4. Jiang, Y., et al. 1992. J. Immunol. 148:2423.
Regulation: Upregulated by IL-1, TNF-α, PDGF, TGF-β
Gene ID: 20296 View all products for this Gene ID 24770 View all products for this Gene ID 6347 View all products for this Gene ID
UniProt: View information about CCL2 on UniProt.org

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