Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining by flow cytometry, the suggested use of this product is ≤ 0.5 ?g per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

ELISA or ELISPOT Capture¹: The Ultra-LEAF? 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. 506002) as the detecting antibody for the detection of mouse MCP-1. The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody (Cat. No. 502609) as the detecting antibody for the detection of human MCP-1.
Flow Cytometry²: The fluorochrome-labeled 2H5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP-1 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization^1,4,6: The Ultra-LEAF? purified antibody (Cat. Nos. 505911 & 505912) is recommended for neutralization.
Additional reported applications (for the relevant formats) include: Western blotting³, immunohistochemistry of paraformaldehyde-fixed, saponin-treated frozen tissue sections⁵, and acetone-fixed frozen tissue sections⁷.

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