Brilliant Violet 421™ anti-mouse CD194 (CCR4
- 产品名称：
- Brilliant Violet 421™ anti-mouse CD194 (CCR4
- 产品类别：
- 抗体
- 产品编号：
- 131217
- 产品应用：
- 131217
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
- Concentration
- ?g size: 0.2 mg/mL?L size: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?l size, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application References
(PubMed link indicates BioLegend citation) -
- Saito K, et al. 2008. J. Immunol. 181:6889. PubMed
- Ueha S, et al. 2007. J. Leukoc. Biol. 82:1230. PubMed
- 3. Sharma R, et al. 2009 J. Immunol. 183:1065 (FC) PubMed
- Dogan R, et al. 2011. J. Leukoc. Biol. 89:93. PubMed
- Liu H, et al. 2011. Sci Transl Med. 3:82. PubMed.
- Shankar SP, et al. 2012. J Immunol. 188:6347. PubMed.
- Product Citations
-
- Xiong Y, et al. 2016. J Immunol. 196: 2526 - 2540. PubMed
- Stathopoulou C, et al. 2020. Immunity. 49(2):247-263.e7.. PubMed
- Herrero–Cervera A, et al. 2019. Diabetologia. 62:2143. PubMed
- Qadir AS, et al. 2021. iScience. 24:103348. PubMed
- Jeljeli M, et al. 2021. Front Immunol. 12:670776. PubMed
- RRID
- AB_10933253 (BioLegend Cat. No. 131217) AB_2650890 (BioLegend Cat. No. 131218)
- Structure
- GPCR, chemokine receptor, seven transmembrane receptor
- Distribution
-
Memory T cells, macrophages, platelets, basophils, Th2 cells, and Tregs
- Function
- CCR4 and its ligands (CCL17 and CCL22) are important for the recruitment of memory T cells into the skin in various cutaneous immune diseases.
- Interaction
- Interaction between CCR4 and its ligand TARC/CCL17 on activated endothelial cells mediates T cell extravasation by stimulating integrin-dependent adhesion
- Ligand/Receptor
- CCl17 and CCL22
- Cell Type
- Basophils, Embryonic Stem Cells, Macrophages, Platelets, T cells, Th2, Tregs
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
-
1. Biederman T, et al. 2002. Eur. J. Immun. 32:3171.
2. Mirshahpanah P, et al. 2008. Exp. Dermatol. 17:30.
3. Kusumoto M, et al. 2007. J. Interferon Cytokine Res 27:901.
4. Clark RA and Kupper TS. 2006. Blood 109:194.
5. Lee I, et al. 2005. J. Exp. Med. 201:1037.
- Gene ID
- 12773 View all products for this Gene ID
- UniProt
- View information about CD194 on UniProt.org
