Ultra-LEAF™ Purified anti-mouse VEGF-A Antib-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3240	1

Product Details

Verified Reactivity

Mouse, Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

N-terminal 24 aa sequence of murine VEGF

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

WB - Quality tested
Neut, ELISA, IHC-F, FA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 ?g/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Neutralization: The Ultra-LEAF? Purified antibody is recommended for neutralization of mouse VEGF-A bioactivity in vivo^2,4 and in vitro².
Western Blotting: It is suggested to use 0.5 - 2 ?g/ml for WB².
Immunohistochemistry: Clone 2G11-2A05 is recommended for IHC on saponin-treated frozen tissue sections at a concentration range of 5-10 ?g/ml.
ELISA or ELISPOT: The Ultra-LEAF? Purified 2G11-2A05 antibody is useful in a mouse VEGF-A ELISA or ELISPOT assay.
Functional Assay: The Ultra-LEAF? Purified 2G11-2A05 antibody is useful in a mouse VEGF-A functional assay².

Application References (PubMed link indicates BioLegend citation)

Wuest TR, et al. 2009. J. Exp Med. 207;101. (Neut) PubMed
Basu A, et al. 2008. Cancer Res. 68:5689. (Neut, WB, FA)
Kumar V, et al. 2010. Blood 115:4725. PubMed
Lu R, et al. 2012. Cancer Res. 72:2239. (Neut) PubMed
Jayarman P, et al. 2012. J. Immunol. 188:5365. PubMed
Kather JN, et al. 2014. Invest Ophthalmol Vis Sci. 55:4295. PubMed

Product Citations

Shi L, et al. 2021. Immunity. . PubMed

RRID

AB_2814439 (BioLegend Cat. No. 512809) AB_2814440 (BioLegend Cat. No. 512810)

Antigen Details

Structure: Cystine-knot growth factors
Distribution: Aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells
Function: Angiogenesis
Cell Targets: Endothelial cells
Receptors: VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1)
Cell Type: Macrophages
Biology Area: Angiogenesis, Cell Biology, Immunology
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Contreras A, et al. 2007. Am. J. Transpl. 7:155.
2. Lambrechts D and Carmeliet P. 2007. Biochim. Biophys. Acta. 1762:1109.
3. Tong JP and Yao YF. 2006. Clin. Biochem. 39:267.
4. Ferrara N, et al. 2003. Nat. Med. 9:669.
5. Shibuya M. 2001. Cell Struct. Funct. 26:25.
6. Senger DR, et al. 1983. Science. 219:983.
Regulation: HIF-1, hypoxiaresponsiveness
Gene ID: 22339 View all products for this Gene ID
UniProt: View information about VEGF-A on UniProt.org

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