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Purified anti-
产品名称:
Purified anti-
产品类别:
抗体
产品编号:
663207
产品应用:
663207
[价格]
规格 价格 库存
25µg ¥ 1344 1

产品详情

Product Details

Verified Reactivity
E. coli, C. trachomatis, M. smegmatis, S. aureus, B. pertussis, R. sphaeroides, V. parahaemolyticus, P. aeruginosa, A. tumefaciens, S. faecalis, A. globiformis
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 2G10 is extremely useful for identifying the major sigma factor from a variety of bacteria. It does not react with Borellia burgdorferi. Clone 2G10 recognizes epitope 470-486.

Additional reported applications (for the relevant formats) include: functional assay and immunoprecipitation.

Application References

(PubMed link indicates BioLegend citation)
  1. Breyer MJ, et al. 1997. J. Bacterial 179:1404.
  2. Lesley SA, et al. 1989. J. Biol. Chem. 264:3794.
  3. Strickland MS, et al. 1988. Biochemistry 27:5755.
Product Citations
  1. Wan T, et al. 2021. Molecular Cell. . PubMed
  2. Kouba T, et al. 2020. Nat Commun. 11:6419. PubMed
  3. Singh KS, et al. 2021. Nature. 589:597. PubMed
RRID
AB_2814498 (BioLegend Cat. No. 663207) AB_2814499 (BioLegend Cat. No. 663208)

Antigen Details

Structure
Sigma 70 (σ70) is a 70 kD transcription factor of E. coli RNA polymerase.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
947567 View all products for this Gene ID
UniProt
View information about Sigma 70 on UniProt.org

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