Direct-Blot™ HRP anti--上海雅吉生物科技有限公司

规格	价格	库存
100µL	￥ 4080	1

Product Details

Verified Reactivity

E. coli, C. trachomatis, M. smegmatis, S. aureus, B. pertussis, R. sphaeroides, V. parahaemolyticus, P. aeruginosa, A. tumefaciens, S. faecalis, A. globiformis

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant protein

Formulation

This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.

Preparation

The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.

Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:4000. The optimal dilution should be determined by titration for each individual assay of interest.

25 ?l and 100 ?l of Direct-Blot? HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

Clone 2G10 is extremely useful for identifying the major sigma factor from a variety of bacteria. It does not react with Borellia burgdorferi. Clone 2G10 recognizes epitope 470-486.

Additional reported applications (for the relevant formats) include: functional assay and immunoprecipitation.

Application References (PubMed link indicates BioLegend citation)

Breyer MJ, et al. 1997. J. Bacterial 179:1404.
Lesley SA, et al. 1989. J. Biol. Chem. 264:3794.
Strickland MS, et al. 1988. Biochemistry 27:5755.

Product Citations

Hughes KJ, et al. 2020. Cell Rep. 33:108527. PubMed
Piel D, et al. 2020. Environ Microbiol. 4198:22. PubMed
Adams DW, et al. 2019. Nat Microbiol. 1.239583333. PubMed
Dar Y, et al. 2022. EMBO Rep. 23:e53981. PubMed
Yang H, et al. 2021. Cell Discov. 7:90. PubMed

RRID

AB_2629596 (BioLegend Cat. No. 663205)

Antigen Details

Structure: Sigma 70 (σ⁷⁰) is a 70 kD transcription factor of E. coli RNA polymerase.
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Gene ID: 947567 View all products for this Gene ID
UniProt: View information about Sigma 70 on UniProt.org

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