- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Syrian Hamster
- Immunogen
- IL-2 activated NK cells from C57BL/6 mice
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
For successful staining of human cells, brighter fluorophore-antibody conjugates, such as PE, BV421?, or APC, are recommended.
Additional reported applications (for relevant formats) include: Western Blotting1.
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Robbins SH, et al. 2002. J. Immunol. 168:2585. (WB)
- Robbins SH, et al. 2003. J. Immunol. 170:5876. (FC)
- McMahon CW, et al. 2002. J. Immunol. 169:1444. (FC)
- Product Citations
-
- Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
- Jaiswal AK, et al. 2020. Immunobiology. 225:151956. PubMed
- Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
- Gupta SS, et al. 2019. Cell Rep. 29:1862. PubMed
- He Y, et al. 2020. Sci Adv. 6:eaba7589. PubMed
- Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed
- Joosten S, et al. 2016. PLoS Pathog. 12: 1005687. PubMed
- Ahrends T, et al. 2017. Immunity. 47:848. PubMed
- She L, et al. 2021. JCI Insight. 6:e143509. PubMed
- Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed
- Pfenninger P, et al. 2022. Front Immunol. 13:777113. PubMed
- Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
- Bharath LP, et al. 2020. Cell Metabolism. 32(1):44-55.e6.. PubMed
- Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed
- Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
- Bourque J, et al. 2021. Heliyon. 7:e08311. PubMed
- Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
- Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
- Gagnon JD, et al. 2019. Cell Rep. 28:2169. PubMed
- Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
- Beura LK, et al. 2018. Immunity. 48:327. PubMed
- Li C, et al. 2018. Cell. 174:285. PubMed
- Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
- Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed
- Chen Y, et al. 2021. Nat Immunol. 22:996. PubMed
- Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
- Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
- Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
- Alwis R, et al. 2016. J Virol. 90: 4771 - 4779. PubMed
- Spath S, et al. 2022. iScience. 25:104998. PubMed
- Zander R, et al. 2020. Immunity. 51(6):1028-1042.e4.. PubMed
- RRID
- AB_2561735 (BioLegend Cat. No. 138415) AB_2561736 (BioLegend Cat. No. 138416)
- Disclaimer
This product may be used for research purposes only.? It is not licensed for resale and may only be used by the buyer.? This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service.? If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California.? The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.
