- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Syrian Hamster
- Immunogen
- IL-2 activated NK cells from C57BL/6 mice
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
For successful staining of human cells, brighter fluorophore-antibody conjugates, such as PE, BV421?, or APC, are recommended.
Additional reported applications (for relevant formats) include: Western Blotting1.
- Application References
(PubMed link indicates BioLegend citation) -
- Robbins SH, et al. 2002. J. Immunol. 168:2585. (WB)
- Robbins SH, et al. 2003. J. Immunol. 170:5876. (FC)
- McMahon CW, et al. 2002. J. Immunol. 169:1444. (FC)
- Product Citations
-
- Moderzynski K, et al. 2016. PLoS Negl Trop Dis. . PubMed
- Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
- Dalmas E et al. 2017. Immunity. 47(5):928-942 . PubMed
- Heindl S, et al. 2021. J Exp Med. 218:. PubMed
- Liu C, et al. 2022. Regen Ther. 21:192. PubMed
- Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
- Burrack AL, et al. 2019. Cell Rep. 28:2140. PubMed
- Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
- Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
- Potluri HK, et al. 2022. J Immunother Cancer. 10:. PubMed
- Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
- Parriott G, et al. 2020. Immunology. 160:280. PubMed
- Zhang Y, et al. 2014. Infect Immun . 82:3033. PubMed
- Emg?rd J, et al. 2018. Immunity. 143:419. PubMed
- Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
- Qi X, et al. 2019. Nat Med. 25:1225. PubMed
- Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
- Zhao H, et al. 2021. Int J Nanomedicine. 16:4913. PubMed
- McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
- Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
- Araujo Furlan CL, et al. 2018. Front Immunol. 2.149305556. PubMed
- Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
- Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
- Schulthei? C, et al. 2020. Immunity. 53(2):442-455.e4.. PubMed
- Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
- Peng V, et al. 2022. Nat Immunol. 23:619. PubMed
- Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed
- Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
- RRID
- AB_10574005 (BioLegend Cat. No. 138407) AB_10574313 (BioLegend Cat. No. 138408)
- Disclaimer
This product may be used for research purposes only.? It is not licensed for resale and may only be used by the buyer.? This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service.? If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California.? The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.
