Brilliant Violet 785™ anti-mouse/human KLRG1
- 产品名称：
- Brilliant Violet 785™ anti-mouse/human KLRG1
- 产品类别：
- 抗体
- 产品编号：
- 138429
- 产品应用：
- 138429
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Syrian Hamster
- Immunogen
- IL-2 activated NK cells from C57BL/6 mice
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785? under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 785? excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
For successful staining of human cells, brighter fluorophore-antibody conjugates, such as PE, BV421?, or APC, are recommended.
Additional reported applications (for relevant formats) include: Western Blotting1.
- Application References
(PubMed link indicates BioLegend citation) -
- Robbins SH, et al. 2002. J. Immunol. 168:2585. (WB)
- Robbins SH, et al. 2003. J. Immunol. 170:5876. (FC)
- McMahon CW, et al. 2002. J. Immunol. 169:1444. (FC)
- Product Citations
-
- Pasciuto E, et al. 2020. Cell. 182:625. PubMed
- Dammeijer F, et al. 2020. Cancer Cell. 38(5):685-700.e8. PubMed
- Jacobs L, et al. 2022. Cancer Gene Ther. 29:984. PubMed
- RRID
- AB_2629749 (BioLegend Cat. No. 138429)
- Structure
- KLRG1 is an inhibitory lectin-like type II transmembrane receptor containing a cytoplasmic motif similar to ITIM, expressed mainly as a homodimeric molecule consisting of two N-glycosylated subunits of approximately 30-38 kD.
- Distribution
-
Subsets of NK cells, lymphokine-activated killer (LAK) cells and subsets of CD8+ and CD4+ cells.
- Function
- Regulates NK cell proliferation, maturation, and homeostasis. Inhibits immune responses by regulating the effector function and the developmental processes of NK and T cells.
- Ligand/Receptor
- E-cadherin
- Cell Type
- NK cells, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Antigen References
-
1. Grundemann C, et al. 2006. J. Immunol. 176:1311.
2. Blaser C, et al. 1998. J. Immunol. 161:6451.
3. Huntington ND, et al. 2007. J. Immunol. 178:4764.
4. Voehringer D, et al. 2001. J. Immunol. 167:4834.
- Gene ID
- 50928 View all products for this Gene ID
- UniProt
- View information about KLRG1 on UniProt.org
