Brilliant Violet 421™ anti-mouse/human KLRG1-上海雅吉生物科技有限公司

规格	价格	库存
125µL	￥ 2448	1

Product Details

Verified Reactivity

Mouse, Human

Antibody Type

Monoclonal

Host Species

Syrian Hamster

Immunogen

IL-2 activated NK cells from C57BL/6 mice

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.

Concentration

?g sizes: 0.2 mg/mL
?L sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested usage of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

For successful staining of human cells, brighter fluorophore-antibody conjugates, such as PE, BV421?, or APC, are recommended.

Additional reported applications (for relevant formats) include: Western Blotting¹.

Application References (PubMed link indicates BioLegend citation)

Robbins SH, et al. 2002. J. Immunol. 168:2585. (WB)
Robbins SH, et al. 2003. J. Immunol. 170:5876. (FC)
McMahon CW, et al. 2002. J. Immunol. 169:1444. (FC)

Product Citations

Hu J, et al. 2022. J Immunother Cancer. 10:. PubMed
Akhmetzyanova I, et al. 2016. J Immunol. 196: 484 - 492. PubMed
Wang D, et al. 2018. Immunity. 48:659. PubMed
Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
Levin MJ, et al. 2018. J Clin Invest. 128:4429. PubMed

RRID

AB_10918627 (BioLegend Cat. No. 138413) AB_2565613 (BioLegend Cat. No. 138414)

Disclaimer

This product may be used for research purposes only.? It is not licensed for resale and may only be used by the buyer.? This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service.? If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California.? The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Antigen Details

Structure: KLRG1 is an inhibitory lectin-like type II transmembrane receptor containing a cytoplasmic motif similar to ITIM, expressed mainly as a homodimeric molecule consisting of two N-glycosylated subunits of approximately 30-38 kD.
Distribution: Subsets of NK cells, lymphokine-activated killer (LAK) cells and subsets of CD8⁺ and CD4⁺ cells.
Function: Regulates NK cell proliferation, maturation, and homeostasis. Inhibits immune responses by regulating the effector function and the developmental processes of NK and T cells.
Ligand/Receptor: E-cadherin
Cell Type: NK cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Antigen References: 1. Grundemann C, et al. 2006. J. Immunol. 176:1311.
2. Blaser C, et al. 1998. J. Immunol. 161:6451.
3. Huntington ND, et al. 2007. J. Immunol. 178:4764.
4. Voehringer D, et al. 2001. J. Immunol. 167:4834.
Gene ID: 50928 View all products for this Gene ID
UniProt: View information about KLRG1 on UniProt.org

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