Purified anti-β-actin Antibody
- 产品名称：
- Purified anti-β-actin Antibody
- 产品类别：
- 抗体
- 产品编号：
- 643801
- 产品应用：
- 643801
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IHC-P - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 ?L per 5 mL antibody dilution buffer for each mini-gel.?For immunocytochemistry, a concentration range of 1.0 - 5.0 ?g/mL is recommended. For immunohistochemistry, a concentration range of 5 - 10 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The binding of this antibody to its target is sensitive to salt concentration. For consistent results, please use?TBS/T buffer for Western blotting that contains 0.15 M NaCl as indicated in the BioLegend recommended protocol.?
- Application References
(PubMed link indicates BioLegend citation) -
- Lawson BR, et al. 2007. J. Immunol. 178:5366.
- Joyce CW, et al. 2006. J. Biol. Chem. 281:33053.
- Yanagiya T,et al.2007.Obesity.15:572.
- KishidaT,et al. 2007.J. Immunol. 179:8554.
- Ouimet M, et al. 2008. Arterisocler Thromb Vasc Biol. 28:1144.
- Toltlt LJ,et al. 2008.J. Immunol. 181:2165.
- Sawada. T,et al. 2008. J. Biol. Chem. 283:26820.
- Ikeda D, et al. 2008. Endocrinology. 149:6037.
- Ikeda Y, et al. 2010. Mol Endocrinol. 24:1338. PubMed
- Kaieda S, et al. 2010. J. Biol Chem. 285:21478. PubMed
- Cambos M, et al. 2011. J. Leukoc. Biol. 89:157. PubMed
- Product Citations
-
- Xu Y, et al. 2022. Nat Commun. 13:980. PubMed
- Greenberg D, et al. 2015. Infect Immun . 83: 4277 - 4292. PubMed
- Sugimoto C, et al. 2022. Elife. 11:. PubMed
- Kaya-Sezginer E, et al. 2022. Andrology. 10:1441. PubMed
- Sims S, et al. 2021. Adv Virol. 2021:5569844. PubMed
- Callahan SM, et al. 2021. Curr Protoc. 1:e294. PubMed
- Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed
- Hadjicharalambous MR, et al. 2019. Sci Rep. 9:6020. PubMed
- Kaieda S, et al. 2010. J Biol Chem. 285:21478. PubMed
- Rodríguez E, et al. 2022. Commun Biol. 5:41. PubMed
- Bruggen R, et al. 2015. PLoS One. 10: 0144555. PubMed
- Budida R, et al. 2017. Eur J Immunol. 47:1819. PubMed
- Hsiao CC, et al. 2021. Cells. 10:. PubMed
- Scorza M 2011. J Leukoc Biol. 89:159. PubMed
- Lovatt M, et al. 2018. Antioxidants (Basel). 0.416666667. PubMed
- Lai CC, et al. 2022. Biomed Rep. 16:19. PubMed
- Kakaradov B, et al. 2017. Nat Immunol. 18:422. PubMed
- Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
- Ikeda Y, et al. 2010. Mol Endocrinol. 24:1338. PubMed
- Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed
- Qin S, et al. 2018. Oncol Lett. 128:1283. PubMed
- Lalor R, et al. 2019. Nutrients. 11. PubMed
- RRID
- AB_2223201 (BioLegend Cat. No. 643801) AB_2223199 (BioLegend Cat. No. 643802)
- Structure
- Actin, cytoplasmic 1
- Distribution
-
Ubiquitously expressed in the cytoplasm of all eukaryotic cells
- Function
- Actins are highly conserved proteins that are involved in cell motility
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microfilaments
- Antigen References
-
1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.
- Gene ID
- 11461 View all products for this Gene ID
- UniProt
- View information about beta-actin on UniProt.org
