Brilliant Violet 785™ anti-mouse CD103 Antib-上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 3432	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Mouse intestinal intraepithelial lymphocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785? under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 785? excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunohistochemical staining^1,7 of acetone-fixed frozen sections, immunofluorescence², and in vitro activation¹.

Application References (PubMed link indicates BioLegend citation)

LeFrancois L, et. al, 1994. Eur. J. Immunol. 24:635. (FC, IHC, IP)
Mysorekar IU, et. al, 2002. J. Biol. Chem. 277:37811. (FC, IF)
Mikami N, et al. 2011. J. Immunol. 186:6886. PubMed
del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed
Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
Xiao B, et al. 2015. PLoS One 1:e0115333. (IHC)

Product Citations

Zhou R, et al. 2022. EBioMedicine. 75:103762. PubMed

RRID

AB_2800588 (BioLegend Cat. No. 121439)

Antigen Details

Structure: Type I transmembrane glycoprotein, Integrin family, can be cleaved into 150 kD and 25 kD chains, associated with β₇ integrin
Distribution: Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells.
Function: Retention and activation of CD103⁺ lymphocytes in the intestinal epithelium, regulate tissue-specific T cell homing.
Ligand/Receptor: E-Cadherin
Cell Type: Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Kilshaw PJ and SJ. Murant. 1990. Eur. J. Immunol. 20:2201.
2. Karecla PI, et al. 1995. Eur. J. Immunol. 25:852.
3. LeFrancois L, et al. 1994. Eur. J. Immunol. 24:635.
4. Sung SS, et al. 2006. J. Immunol. 176:2161.
5. Johansson-Lindbom B, et al. 2005. J. Exp. Med. 202:1063.
6. Dujardin HC, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:14473.
Gene ID: 16407 View all products for this Gene ID
UniProt: View information about CD103 on UniProt.org

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