Alexa Fluor® 700 anti-mouse CD103 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1692	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Mouse intestinal intraepithelial lymphocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunohistochemical staining^1,7 of acetone-fixed frozen sections, immunofluorescence², and in vitro activation¹.

Application References (PubMed link indicates BioLegend citation)

LeFrancois L, et. al, 1994. Eur. J. Immunol. 24:635. (FC, IHC, IP)
Mysorekar IU, et. al, 2002. J. Biol. Chem. 277:37811. (FC, IF)
Mikami N, et al. 2011. J. Immunol. 186:6886. PubMed
del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed
Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
Xiao B, et al. 2015. PLoS One 1:e0115333. (IHC)

Product Citations

Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed

RRID

AB_2813992 (BioLegend Cat. No. 121441) AB_2813993 (BioLegend Cat. No. 121442)

Antigen Details

Structure: Type I transmembrane glycoprotein, Integrin family, can be cleaved into 150 kD and 25 kD chains, associated with β₇ integrin
Distribution: Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells.
Function: Retention and activation of CD103⁺ lymphocytes in the intestinal epithelium, regulate tissue-specific T cell homing.
Ligand/Receptor: E-Cadherin
Cell Type: Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Kilshaw PJ and SJ. Murant. 1990. Eur. J. Immunol. 20:2201.
2. Karecla PI, et al. 1995. Eur. J. Immunol. 25:852.
3. LeFrancois L, et al. 1994. Eur. J. Immunol. 24:635.
4. Sung SS, et al. 2006. J. Immunol. 176:2161.
5. Johansson-Lindbom B, et al. 2005. J. Exp. Med. 202:1063.
6. Dujardin HC, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:14473.
Gene ID: 16407 View all products for this Gene ID
UniProt: View information about CD103 on UniProt.org

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