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首页 > 抗体蛋白 > 抗体
Alexa Fluor® 647 anti-mouse CD103 Antibody
产品名称：
Alexa Fluor® 647 anti-mouse CD103 Antibody
产品类别：
抗体
产品编号：
121409
产品应用：
121409
[价格]
规格 价格 库存
25µg ￥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse intestinal intraepithelial lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining1,7 of acetone-fixed frozen sections, immunofluorescence2, and in vitro activation1.

Application References

(PubMed link indicates BioLegend citation)
  1. LeFrancois L, et. al, 1994. Eur. J. Immunol. 24:635. (FC, IHC, IP)
  2. Mysorekar IU, et. al, 2002. J. Biol. Chem. 277:37811. (FC, IF)
  3. Mikami N, et al. 2011. J. Immunol. 186:6886. PubMed
  4. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  5. Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed
  6. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
  7. Xiao B, et al. 2015. PLoS One 1:e0115333. (IHC)
Product Citations
  1. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  2. van Wijck Y, et al. 2018. J Immunol. 200:1570. PubMed
  3. Vella JL, et al. 2021. Life Sci Alliance. 4:. PubMed
  4. Wang D, et al. 2018. Immunity. 48:659. PubMed
  5. Lu J, et al. 2018. ACS Nano. 12:11041. PubMed
  6. Ihara S, et al. 2022. PLoS Negl Trop Dis. 16:e0010323. PubMed
  7. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
  8. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  9. Hao X, et al. 2008. J Virol. 82:4908. PubMed
  10. Dewi IMW, et al. 2021. Cell Reports Medicine. 2(5):100289. PubMed
  11. Hilpert C, et al. 2019. J Immunol. 203:3068. PubMed
  12. López MJ, et al. 2018. Invest Ophthalmol Vis Sci. 59:5671. PubMed
  13. Schmitt E, et al. 2012. J Immunol. 186:5683. PubMed
  14. Lam KC, et al. 2021. Cell. 184:5338. PubMed
  15. Kim T, Braciale T 2009. PLoS One. 4:e4204. PubMed
RRID
AB_535951 (BioLegend Cat. No. 121409) AB_535952 (BioLegend Cat. No. 121410)

Antigen Details

Structure
Type I transmembrane glycoprotein, Integrin family, can be cleaved into 150 kD and 25 kD chains, associated with β7 integrin
Distribution

Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells.

Function
Retention and activation of CD103+ lymphocytes in the intestinal epithelium, regulate tissue-specific T cell homing.
Ligand/Receptor
E-Cadherin
Cell Type
Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kilshaw PJ and SJ. Murant. 1990. Eur. J. Immunol. 20:2201.
2. Karecla PI, et al. 1995. Eur. J. Immunol. 25:852.
3. LeFrancois L, et al. 1994. Eur. J. Immunol. 24:635.
4. Sung SS, et al. 2006. J. Immunol. 176:2161.
5. Johansson-Lindbom B, et al. 2005. J. Exp. Med. 202:1063.
6. Dujardin HC, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:14473.

Gene ID
16407 View all products for this Gene ID
UniProt
View information about CD103 on UniProt.org

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