Alexa Fluor® 647 anti-IDO1 Antibody
- 产品名称：
- Alexa Fluor® 647 anti-IDO1 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 654003
- 产品应用：
- 654003
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant mouse IDO1.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Clone 2E2/IDO1 reacts with mouse IDO1. It does not appear to react with mouse IDO2.
- Product Citations
-
- Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
- Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
- Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
- Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed
- Li A, et al. 2018. Cancer Lett. 431:54. PubMed
- RRID
- AB_2564584 (BioLegend Cat. No. 654003) AB_2564585 (BioLegend Cat. No. 654004)
- Structure
- 403 amino acids with a predicted molecular weight of 45 kD.
- Distribution
-
Cytoplasm
- Function
- Indoleamine 2,3-dioxygenase (IDO) is an IFN-γ inducible gene. It catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated to protect against intracellular and extracellular pathogens.
- Interaction
- This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin.
- Biology Area
- Cell Biology, Immunology
- Antigen References
-
1. Habara-Ohkubo A, et al. 1991. Gene 105:221.
2. Munn DH, et al. 2002. Science 297:1867.
3. Frumento G, et al. 2002. J. Exp. Med. 196:459.
4. Muller AJ, et al. 2005. Nature Med. 11:312.
5. Lob S, et al. 2009. Nature Rev. Cancer 9:445.
- Gene ID
- 15930 View all products for this Gene ID
- UniProt
- View information about IDO1 on UniProt.org
