Purified anti-mouse CD119 (IFN-γ R α c
- 产品名称:
- Purified anti-mouse CD119 (IFN-γ R α c
- 产品类别:
- 抗体
- 产品编号:
- 112802
- 产品应用:
- 112802
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Soluble recombinant mouse IFN-γR α chain protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IP, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting and immunoprecipitation1. The 2E2 antibody does not block the binding of IFN-γ to IFN-γ receptor. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112804) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).
- Application References
(PubMed link indicates BioLegend citation) -
- Bach EA, et al. 1995. Science 270:1215.
- Product Citations
-
- Liu XD, et al. 2020. Nat Commun. 11:2135. PubMed
- RRID
- AB_2123477 (BioLegend Cat. No. 112802)
- Structure
- Ig superfamily, class II cytokine receptor family, associates with IFN-γ AF-1, 90 kD
- Distribution
-
T cells and B cells, NK cells, myeloid cells, fibroblasts, endothelial and epithelial cells
- Function
- Initiates and regulates immune response
- Ligand/Receptor
- IFN-γ
- Cell Type
- B cells, Endothelial cells, Epithelial cells, Fibroblasts, NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Basu M, et al. 1988. P. Natl. Acad. Sci. USA 85:6282.
2. Codias EK, et al. 1990. J. Immunol. 144:2197.
- Gene ID
- 15979 View all products for this Gene ID
- UniProt
- View information about CD119 on UniProt.org