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Purified anti-mouse CD119 (IFN-γ R α c
产品名称:
Purified anti-mouse CD119 (IFN-γ R α c
产品类别:
抗体
产品编号:
112802
产品应用:
112802
[价格]
规格 价格 库存
500µg ¥ 2964 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Soluble recombinant mouse IFN-γR α chain protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting and immunoprecipitation1. The 2E2 antibody does not block the binding of IFN-γ to IFN-γ receptor. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112804) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Bach EA, et al. 1995. Science 270:1215.
Product Citations
  1. Liu XD, et al. 2020. Nat Commun. 11:2135. PubMed
RRID
AB_2123477 (BioLegend Cat. No. 112802)

Antigen Details

Structure
Ig superfamily, class II cytokine receptor family, associates with IFN-γ AF-1, 90 kD
Distribution

T cells and B cells, NK cells, myeloid cells, fibroblasts, endothelial and epithelial cells

Function
Initiates and regulates immune response
Ligand/Receptor
IFN-γ
Cell Type
B cells, Endothelial cells, Epithelial cells, Fibroblasts, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Basu M, et al. 1988. P. Natl. Acad. Sci. USA 85:6282.
2. Codias EK, et al. 1990. J. Immunol. 144:2197.

Gene ID
15979 View all products for this Gene ID
UniProt
View information about CD119 on UniProt.org

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