Biotin anti-mouse CD119 (IFN-γ R α cha
- 产品名称：
- Biotin anti-mouse CD119 (IFN-γ R α cha
- 产品类别：
- 抗体
- 产品编号：
- 112803
- 产品应用：
- 112803
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Soluble recombinant mouse IFN-γR α chain protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting and immunoprecipitation1. The 2E2 antibody does not block the binding of IFN-γ to IFN-γ receptor. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112804) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).
- Application References
(PubMed link indicates BioLegend citation) -
- Bach EA, et al. 1995. Science 270:1215.
- Cella M, et al. 2010. Proc Natl Acad Sci USA. 107:10961. PubMed
- de Bruin AM, et al. 2010. Blood 116:2559. PubMed
- Product Citations
-
- Kiritsy MC, et al. 2021. Elife. 10:. PubMed
- Bruin A, et al. 2010. Blood. 116:2559. PubMed
- Cella M, et al. 2010. Proc Natl Acad Sci U S A. 107:10961. PubMed
- Ji L et al. 2019. Immunity. 51(2):272-284 . PubMed
- DanDan Huang, Lang Bao 2016. J Microbiol Immunol Infect. 49:859-865. PubMed
- Ribechini E, et al. 2017. Blood Adv. 0.699305556. PubMed
- RRID
- AB_2123476 (BioLegend Cat. No. 112803) AB_2123475 (BioLegend Cat. No. 112804)
- Structure
- Ig superfamily, class II cytokine receptor family, associates with IFN-γ AF-1, 90 kD
- Distribution
- T cells and B cells, NK cells, myeloid cells, fibroblasts, endothelial and epithelial cells
- Function
- Initiates and regulates immune response
- Ligand/Receptor
- IFN-γ
- Cell Type
- T cells, B cells, NK cells, Fibroblasts, Endothelial cells, Epithelial cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Basu M, et al. 1988. P. Natl. Acad. Sci. USA 85:6282.
2. Codias EK, et al. 1990. J. Immunol. 144:2197.
- Gene ID
- 15979 View all products for this Gene ID
- UniProt
- View information about CD119 on UniProt.org
