Purified anti-GFAP Antibody
- 产品名称：
- Purified anti-GFAP Antibody
- 产品类别：
- 抗体
- 产品编号：
- 644701
- 产品应用：
- 644701
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Bovine spinal cord homogenate
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IHC-F - Verified
FC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.25 - 1.0 ?g antibody per mL?antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 5.0 ?g/mL?is recommended. For immunohistochemical staining of frozen tissue sections, a concentration range of 5.0 - 10 ?g/mL?is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
(PubMed link indicates BioLegend citation) -
- McLendon RE and Bigner DD. 1994. Brain Pathol. 4:221.
- Liu W, et al. 2011. Proteomics 11:3556. (FC) PubMed
- Product Citations
-
- Tao W, et al. 2020. EMBO Mol Med. 12:e12291. PubMed
- Eisemann T, et al. 2018. BMC Cancer. 18:103. PubMed
- Liu CL, et al. 2018. Mol Med Rep. 9:2359. PubMed
- Gerasimov E, et al. 2021. Int J Mol Sci. 22:. PubMed
- Shi Y, et al. 2016. Cell Death Differ. 10.1038/cdd.2016.110. PubMed
- Gómez-Gálvez Y, et al. 2020. J Neurosci Res. 1417:98. PubMed
- Ivanova EL, et al. 2022. Front Oncol. 12:969787. PubMed
- Costa B, et al. 2021. Cancers (Basel). 13:00. PubMed
- Moyon S, et al. 2021. Nature Communications. 12(1):3359. PubMed
- Marcelo A, et al. 2021. Cell Death Dis. 12:1117. PubMed
- Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed
- Chompre G, et al. 2022. Heliyon. 8:e11194. PubMed
- Murenu E, et al. 2021. Int J Mol Sci. 22:. PubMed
- Costa B, et al. 2019. Blood Adv. 3:1092. PubMed
- RRID
- AB_2109791 (BioLegend Cat. No. 644701) AB_2294566 (BioLegend Cat. No. 644702)
- Structure
- Around 50 kD
- Distribution
-
Only expressed in astrocytes in the central nervous system.
- Function
- Together with other proteins to form intermediate filaments which supports astroglial cells. Astroglial cells support and nourish cells in the brain and spinal cord. If brain cells are injured through trauma or disease, astroglial cells react by rapidly prodcing more GFAP protein.
- Cell Type
- Astrocytes
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Gene ID
- 2670 View all products for this Gene ID
- UniProt
- View information about GFAP on UniProt.org
