Alexa Fluor® 488 anti-Bax Antibody
- 产品名称：
- Alexa Fluor® 488 anti-Bax Antibody
- 产品类别：
- 抗体
- 产品编号：
- 633603
- 产品应用：
- 633603
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Amino acids 3-16 of human Bax protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our?True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Clone 2D2 has been shown to be useful for Western blotting1, immunoprecipitation2, immunofluorescence3 and immunohistochemical staining3 of formalin-fixed and paraffin-embedded tissue sections. This antibody does not cross-react with Bcl-2 or Bcl-XL proteins.
Clone 2D2 does not show specificity for mouse BAX.
- Application References
(PubMed link indicates BioLegend citation) -
- Hsu YT, et al. 1997. J. Biol. Chem. 272:13829. (WB)
- Cartron PF, et al. 2004. FEBS Lett. 578:41. (IP)
- Pucci S, et al. 2009. Cell Cycle 8:473. (IF, IHC)
- Product Citations
-
- Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
- Lin J, Sorger M 2015. Sci Rep. 6: 8390. PubMed
- Herrera FG, et al. 2019. Int J Radiat Oncol Biol Phys. 103:320. PubMed
- RRID
- AB_2562171 (BioLegend Cat. No. 633603) AB_2562172 (BioLegend Cat. No. 633604)
- Structure
- Forms homodimers and heterodimers with Bcl-2, approximately 21 kD
- Distribution
-
Cytoplasm, mitochondria, nucleus; expressed in hematopoietic stem cells, ovaries, lymph nodes
- Function
- Induces cell death through the process of apoptosis by acting on mitochondria
- Interaction
- 14-3-3 theta, VDAC1, Bcl-2, solute carrier family 25, mitochondrial carrier member 4
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
- Antigen References
-
1. LeBlanc H, et al. 2002. Nat. Med. 8:274.
2. Marzo I, et al. 1998. Science 281:2027.
3. Miyashita T et al. 1995. Cell 80:293.
4. Oltvai ZN, et al. 1993. Cell 74:609.
- Gene ID
- 581 View all products for this Gene ID
- UniProt
- View information about Bax on UniProt.org
