PerCP/Cyanine5.5 anti-human CD80 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1416	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of T cell activation, immunohistochemical staining of acetone-fixed frozen tissue sections², immunoprecipitation, and Western blotting³. The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 305245 & 305246).

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
Battifora M. 1998. J. Clin. Endocr. Metab. 83:4130. (IHC)
Van der Merwe PA, et al. 1997. J. Exp. Med. 185:3. (WB)
Jayakumar A, et al. 2008. Infect. Immun. 76:2138. PubMed
Schubert DA, et al. 2012. J. Exp Med. 209:335. PubMed
Wen T, et al. 2014. J Immunol. 192:5481. PubMed

Product Citations

Hsu SPC, et al. 2020. J Neurooncol. 146:417. PubMed
Manukyan G, et al. 2020. Auto Immun Highlights. 11:5. PubMed
Israelow B, et al. 2021. Sci Immunol. 6:eabl4509. PubMed
Flieswasser T, et al. 2020. Cells. 9:00. PubMed
Yu X, et al. 2020. Cancer Cell. 37(6):850-866. PubMed
Shrestha P, et al. 2021. PLoS Pathog. 17:e1009091. PubMed
Krakow S, et al. 2019. Front Immunol. 10:1484. PubMed
Pi J, et al. 2022. J Nanobiotechnology. 20:36. PubMed
Riegel K, et al. 2021. Cell Death Dis. 12:969. PubMed
Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed

RRID

AB_2566490 (BioLegend Cat. No. 305231) AB_2566491 (BioLegend Cat. No. 305232)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, 60 kD
Distribution: Activated B cells and T cells, macrophages, and dendritic cells
Function: T cell costimulation
Ligand/Receptor: CD28, CD152 (CTLA-4)
Cell Type: B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Freeman G, et al. 1991. J. Exp. Med. 174:625.
2. Linsley P, et al. 1996. Immunity 4:535.
3. Linsley P, et al. 1991. J. Exp. Med. 174:561.
Gene ID: 941 View all products for this Gene ID
UniProt: View information about CD80 on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们