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首页 > 抗体蛋白 > 抗体
PE/Cyanine7 anti-human CD80 Antibody
产品名称：
PE/Cyanine7 anti-human CD80 Antibody
产品类别：
抗体
产品编号：
305217
产品应用：
305217
[价格]
规格 价格 库存
25tests ￥ 1692 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of T cell activation, immunohistochemical staining of acetone-fixed frozen tissue sections2, immunoprecipitation, and Western blotting3. The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 305245 & 305246).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Battifora M. 1998. J. Clin. Endocr. Metab. 83:4130. (IHC)
  3. Van der Merwe PA, et al. 1997. J. Exp. Med. 185:3. (WB)
  4. Jayakumar A, et al. 2008. Infect. Immun. 76:2138. PubMed
  5. Schubert DA, et al. 2012. J. Exp Med. 209:335. PubMed
  6. Wen T, et al. 2014. J Immunol. 192:5481. PubMed
Product Citations
  1. Magalh?es L, et al. 2015. PLoS Negl Trop Dis. 10: 0003816. PubMed
  2. Zhou R, et al. 2020. Immunity. S1074-7613(20)30333-2.. PubMed
  3. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  4. Abe S, et al. 2020. JCI Insight. 5:00. PubMed
  5. Meng Y,et al. 2017. Cell Death Dis. . 10.1038/cddis.2017.505. PubMed
  6. Kotsiou E, et al. 2016. Blood. 128: 72 - 81. PubMed
  7. Neff CP et al. 2018. EBioMedicine. 30:192-202 . PubMed
  8. Cassetta L et al. 2019. Cancer Cell. 35(4):588-602 . PubMed
  9. Sarkar MK, et al. 2018. Ann Rheum Dis. 77:1653. PubMed
  10. Boyd A, et al. 2013. Infect Immun . 81:1420. PubMed
  11. Sureshchandra S, et al. 2021. iScience. 24:102690. PubMed
  12. Sabree SA, et al. 2021. J Immunother Cancer. 9: . PubMed
  13. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  14. Richardson JR, et al. 2018. Front Immunol. 2.182638889. PubMed
  15. Cecotto L, et al. 2022. iScience. 25:105211. PubMed
  16. Koh WH, et al. 2020. STAR Protoc. 1:100203. PubMed
  17. Murphy DM, et al. 2022. J Clin Invest. Online ahead of print. PubMed
RRID
AB_1877254 (BioLegend Cat. No. 305217) AB_2076148 (BioLegend Cat. No. 305218)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 60 kD
Distribution

Activated B cells and T cells, macrophages, and dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Freeman G, et al. 1991. J. Exp. Med. 174:625.
2. Linsley P, et al. 1996. Immunity 4:535.
3. Linsley P, et al. 1991. J. Exp. Med. 174:561.

Gene ID
941 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

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