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PE anti-human CD80 Antibody
产品名称：
PE anti-human CD80 Antibody
产品类别：
抗体
产品编号：
305207
产品应用：
305207
[价格]
规格 价格 库存
25tests ￥ 1188 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of T cell activation, immunohistochemical staining of acetone-fixed frozen tissue sections2, immunoprecipitation, and Western blotting3. The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 305245 & 305246).

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Battifora M. 1998. J. Clin. Endocr. Metab. 83:4130. (IHC)
  3. Van der Merwe PA, et al. 1997. J. Exp. Med. 185:3. (WB)
  4. Jayakumar A, et al. 2008. Infect. Immun. 76:2138. PubMed
  5. Schubert DA, et al. 2012. J. Exp Med. 209:335. PubMed
  6. Wen T, et al. 2014. J Immunol. 192:5481. PubMed
Product Citations
  1. Werz O, et al. 2018. Nat Commun. 9:59. PubMed
  2. Lathrop S, et al. 2015. Infect Immun . 83: 2661 - 2671. PubMed
  3. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  4. Hagan T, et al. 2020. Cell. 178(6):1313-1328.e13.. PubMed
  5. Sugawara E, et al. 2020. Autophagy. 1.323611111. PubMed
  6. Rosskopf S, et al. 2016. Sci Rep. 6:31580. PubMed
  7. Hou ZH, et al. 2020. Am J Physiol Cell Physiol. 318:C649. PubMed
  8. Santiago K, et al. 2016. Pathog Dis. 74: ftw081. PubMed
  9. Du X, et al. 2018. Cell Res. 28:416. PubMed
  10. Herath S, et al. 2014. PLoS One. 9:88327. PubMed
  11. Wang D, et al. 2016. J Cell Sci. 129: 2261 - 2272. PubMed
  12. Guo Z, et al. 2022. NPJ Precis Oncol. 6:34. PubMed
  13. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  14. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  15. Pellegrini JM, et al. 2021. Sci Rep. 11:13559. PubMed
  16. Lewis SA, et al. 2021. Front Immunol. 12:724015. PubMed
  17. Balan I, et al. 2022. Front Immunol. 13:940095. PubMed
  18. Ganesan R, et al. 2020. FASEB J. 34:15888. PubMed
  19. Zhang L, et al. 2011. Blood. 117:6184. PubMed
  20. Blaszczak AM, et al. 2019. J Diabetes Res. 2019:8124563. PubMed
  21. Serhan J 2012. Blood. 120:e60. PubMed
  22. Nowak W, et al. 2020. EBioMedicine. 50:290-305.. PubMed
  23. Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed
  24. Luque-Martin R, et al. 2021. J Immunol. 207:555. PubMed
  25. Pi J, et al. 2022. J Nanobiotechnology. 20:36. PubMed
  26. Kubala MH, et al. 2018. Cell Rep. 25:2177. PubMed
  27. G?rtner K, et al. 2019. J Extracell Vesicles. 8:1573051. PubMed
  28. Longbrake EE, et al. 2018. Mult Scler. 24:728. PubMed
  29. Corgnac S, et al. 2022. J Immunother Cancer. 10:. PubMed
  30. Chen R, et al. 2021. Front Oncol. 11:743050. PubMed
  31. Morell M, et al. 2022. EBioMedicine. 76:103808. PubMed
  32. Trabanelli S, et al. 2014. J Immunol. 192:1231. PubMed
RRID
AB_314503 (BioLegend Cat. No. 305207) AB_314504 (BioLegend Cat. No. 305208)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 60 kD
Distribution

Activated B cells and T cells, macrophages, and dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Freeman G, et al. 1991. J. Exp. Med. 174:625.
2. Linsley P, et al. 1996. Immunity 4:535.
3. Linsley P, et al. 1991. J. Exp. Med. 174:561.

Gene ID
941 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

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