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首页 > 抗体蛋白 > 抗体
FITC anti-human CD80 Antibody
产品名称：
FITC anti-human CD80 Antibody
产品类别：
抗体
产品编号：
305205
产品应用：
305205
[价格]
规格 价格 库存
25tests ￥ 996 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of T cell activation, immunohistochemical staining of acetone-fixed frozen tissue sections2, immunoprecipitation, and Western blotting3. The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 305245 & 305246).

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Battifora M. 1998. J. Clin. Endocr. Metab. 83:4130. (IHC)
  3. Van der Merwe PA, et al. 1997. J. Exp. Med. 185:3. (WB)
  4. Jayakumar A, et al. 2008. Infect. Immun. 76:2138. PubMed
  5. Schubert DA, et al. 2012. J. Exp Med. 209:335. PubMed
  6. Wen T, et al. 2014. J Immunol. 192:5481. PubMed
Product Citations
  1. Bauer J, et al. 2022. Nat Commun. 13:6401. PubMed
  2. Kan S, et al. 2020. Int J Oncol. 57:1047. PubMed
  3. Wang X, et al. 2021. Front Physiol. 12:644903. PubMed
  4. Miao L, et al. 2021. Clin Transl Med. 11:e395. PubMed
  5. Marn Franco JL, et al. 2020. Cell Reports. 33(13):108547. PubMed
  6. Eken A, et al. 2020. North Clin Istanb. 0.513194444. PubMed
  7. Lin H, et al. 2020. Small. 16:e2002194. PubMed
  8. Du B, et al. 2020. Scand J Immunol. 91:e12872. PubMed
  9. Liu Y, et al. 2020. Oncol Lett. 2369:20. PubMed
  10. Serhan J 2012. Blood. 120:e60. PubMed
  11. Denniston A, et al. 2012. Invest Ophthalmol Vis Sci. 53:888. PubMed
  12. Denniston A, et al. 2011. J Immunol. 186:305. PubMed
  13. Li X, et al. 2021. Cell Death Dis. 12:314. PubMed
  14. Wang J, et al. 2022. Oncoimmunology. 11:2016159. PubMed
  15. Milillo MA, et al. 2019. Front Immunol. 10:2181. PubMed
  16. Riegel K, et al. 2021. Cell Death Dis. 12:969. PubMed
  17. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  18. Agod Z, et al. 2017. Redox Biol. 10.1016/j.redox.2017.07.016. PubMed
  19. Rusconi M, et al. 2017. Sci Rep. 5.745138889. PubMed
  20. Ronaghan NJ, et al. 2022. PLoS One. 17:e0276013. PubMed
RRID
AB_314501 (BioLegend Cat. No. 305205) AB_314502 (BioLegend Cat. No. 305206)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 60 kD
Distribution

Activated B cells and T cells, macrophages, and dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Freeman G, et al. 1991. J. Exp. Med. 174:625.
2. Linsley P, et al. 1996. Immunity 4:535.
3. Linsley P, et al. 1991. J. Exp. Med. 174:561.

Gene ID
941 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

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