首页 > 抗体蛋白 > 抗体
Biotin anti-human CD80 Antibody
产品名称:
Biotin anti-human CD80 Antibody
产品类别:
抗体
产品编号:
305204
产品应用:
305204
[价格]
规格 价格 库存
100µg ¥ 2256 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of T cell activation, immunohistochemical staining of acetone-fixed frozen tissue sections2, immunoprecipitation, and Western blotting3. The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 305245 & 305246).

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Battifora M. 1998. J. Clin. Endocr. Metab. 83:4130. (IHC)
  3. Van der Merwe PA, et al. 1997. J. Exp. Med. 185:3. (WB)
  4. Jayakumar A, et al. 2008. Infect. Immun. 76:2138. PubMed
  5. Schubert DA, et al. 2012. J. Exp Med. 209:335. PubMed
  6. Wen T, et al. 2014. J Immunol. 192:5481. PubMed
Product Citations
  1. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
RRID
AB_314500 (BioLegend Cat. No. 305204)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 60 kD
Distribution

Activated B cells and T cells, macrophages, and dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Freeman G, et al. 1991. J. Exp. Med. 174:625.
2. Linsley P, et al. 1996. Immunity 4:535.
3. Linsley P, et al. 1991. J. Exp. Med. 174:561.

Gene ID
941 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

联系我们

TEL:021-34661275  点击拨打热线