Alexa Fluor® 647 anti-human CD80 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1416	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of T cell activation, immunohistochemical staining of acetone-fixed frozen tissue sections², immunoprecipitation, and Western blotting³. The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 305245 & 305246).

Application References (PubMed link indicates BioLegend citation)

Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
Battifora M. 1998. J. Clin. Endocr. Metab. 83:4130. (IHC)
Van der Merwe PA, et al. 1997. J. Exp. Med. 185:3. (WB)
Jayakumar A, et al. 2008. Infect. Immun. 76:2138. PubMed
Schubert DA, et al. 2012. J. Exp Med. 209:335. PubMed
Wen T, et al. 2014. J Immunol. 192:5481. PubMed

Product Citations

Frensch M, et al. 2021. Cell Mol Life Sci. 78:8165. PubMed
Wu Y, et al. 2022. J Cancer Res Clin Oncol. . PubMed
Navarro-Guerrero E, et al. 2021. Sci Rep. 11:4245. PubMed

RRID

AB_528874 (BioLegend Cat. No. 305215) AB_528875 (BioLegend Cat. No. 305216)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, 60 kD
Distribution: Activated B cells and T cells, macrophages, and dendritic cells
Function: T cell costimulation
Ligand/Receptor: CD28, CD152 (CTLA-4)
Cell Type: B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Freeman G, et al. 1991. J. Exp. Med. 174:625.
2. Linsley P, et al. 1996. Immunity 4:535.
3. Linsley P, et al. 1991. J. Exp. Med. 174:561.
Gene ID: 941 View all products for this Gene ID
UniProt: View information about CD80 on UniProt.org

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