Purified anti-human CD45 Antibody
- 产品名称：
- Purified anti-human CD45 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 368502
- 产品应用：
- 368502
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human PBMC
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-P - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
- Application References
(PubMed link indicates BioLegend citation) -
- Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33.
- Csiba A, et al. 1984. Br. J. Cancer 50:699.
- Tchilian EZ, et al. 2001. J. Immunol. 166:1308.
- Lee MS, et al. 2004. Int. Immunol. 16:1109.
- Product Citations
-
- Korn MA, et al. 2020. J Immunol. 205:2595. PubMed
- Wang J, et al. 2020. Sci Adv. 6:eaax3223. PubMed
- Mann ER, et al. 2020. Sci Immunol. :5. PubMed
- Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
- Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
- Walker DG, et al. 2019. Cells. 8. PubMed
- Cobb DA, et al. 2022. J Immunother Cancer. 10:. PubMed
- Xie S, et al. 2020. Ann Transl Med. 1.384027778. PubMed
- Balakrishnan A, et al. 2019. Sci Rep. 9:7933. PubMed
- Donnelly C, et al. 2018. Sci Rep. 0.625. PubMed
- W?ldchen F, et al. 2020. Nat Commun. 1.074305556. PubMed
- Ran LJ, et al. 2018. Mol Med Rep. 18:723. PubMed
- RRID
- AB_2566240 (BioLegend Cat. No. 368502)
- Structure
- Type I transmembrane protein, 180 - 240 kD (multiple isoforms)
- Distribution
-
Hematopoietic cells, except circulating erythrocytes or platelets.
- Function
- Tyrosine phosphatases, signaling, co-stimulation (co-inhibition), TCR and BCR mediated activation.
- Ligand/Receptor
- Galectin-1, CD2, CD3, and CD4.
- Cell Type
- B cells, Dendritic cells, Neutrophils
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Protein Kinases/Phosphatase, TCRs
- Antigen References
-
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45 on UniProt.org
