PerCP/Cyanine5.5 anti-human CD45 Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-human CD45 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 368503
- 产品应用：
- 368503
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1764 |
1 |
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human PBMC
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33.
- Csiba A, et al. 1984. Br. J. Cancer 50:699.
- Tchilian EZ, et al. 2001. J. Immunol. 166:1308.
- Lee MS, et al. 2004. Int. Immunol. 16:1109.
- Product Citations
-
- Zaman R, et al. 2021. Immunity. :. PubMed
- Fujino S, et al. 2021. Mol Cancer Res. 19:812. PubMed
- Gao Q, et al. 2019. J Exp Med. 216:688. PubMed
- Eri Yamada et al. 2018. Cell host & microbe. 23(1):110-120 . PubMed
- Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed
- Slingsby MHL, et al. 2021. Haematologica. Online ahead of print. PubMed
- Hu G, et al. 2022. Cell Death Dis. 13:640. PubMed
- Appios A, et al. 2021. Bio Protoc. 11:e4044. PubMed
- Xiang W, et al. 2018. Nat Commun. 9:2574. PubMed
- Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
- Ramond C et al. 2017. eLife. 6 pii: e27564. PubMed
- Wang X, et al. 2021. Immunity. 54(6):1123-1136.e8. PubMed
- RRID
- AB_2566351 (BioLegend Cat. No. 368503) AB_2566352 (BioLegend Cat. No. 368504)
- Structure
- Type I transmembrane protein, 180 - 240 kD (multiple isoforms)
- Distribution
-
Hematopoietic cells, except circulating erythrocytes or platelets.
- Function
- Tyrosine phosphatases, signaling, co-stimulation (co-inhibition), TCR and BCR mediated activation.
- Ligand/Receptor
- Galectin-1, CD2, CD3, and CD4.
- Cell Type
- B cells, Dendritic cells, Neutrophils
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Protein Kinases/Phosphatase, TCRs
- Antigen References
-
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45 on UniProt.org
