PE anti-human CD45 Antibody
- 产品名称：
- PE anti-human CD45 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 368509
- 产品应用：
- 368509
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human PBMC
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
- Application References
(PubMed link indicates BioLegend citation) -
- Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33.
- Csiba A, et al. 1984. Br. J. Cancer 50:699.
- Tchilian EZ, et al. 2001. J. Immunol. 166:1308.
- Lee MS, et al. 2004. Int. Immunol. 16:1109.
- Product Citations
-
- Sonoda S, et al. 2022. STAR Protoc. 3:101386. PubMed
- Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
- Sluzalska K,et al. 2017. Sci Rep.. 10.1038/s41598-017-14004-9. PubMed
- Delvecchio FR, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1543. PubMed
- Dam T, et al. 2021. Front Physiol. 11:613367. PubMed
- Sluzalska KD, et al. 2017. Osteoarthritis Cartilage. 25:1890. PubMed
- Zhuang J, et al. 2020. Nucleic Acids Res. 48:8870. PubMed
- Morton JJ, et al. 2018. Mol Carcinog. 57:1651. PubMed
- Ali S, et al. 2018. Purinergic Signal. 14:371. PubMed
- Zhang X, et al. 2022. Nat Commun. 13:5211. PubMed
- Li Y, et al. 2021. Aging (Albany NY). 13:25886. PubMed
- Chan JM, et al. 2021. Cancer Cell. 39:1479. PubMed
- Cillo AR, et al. 2020. Immunity. 52(1):183-199.e9.. PubMed
- Liu Y, et al. 2022. iScience. 25:104405. PubMed
- Yu C, et al. 2020. Sci Rep. 10:14521. PubMed
- Yu LC, et al. 2020. Front Neurol. 1.104166667. PubMed
- Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed
- Rai MA, et al. 2020. Retrovirology. 17:35. PubMed
- Tidu F, et al. 2021. iScience. 24(6):102683. PubMed
- Zhang Y, et al. 2018. Stem Cells Int. 2018:7159465. PubMed
- Chioh FW, et al. 2021. eLife. 10:00. PubMed
- Yao X, et al. 2022. Bioeng Transl Med. 7:e10250. PubMed
- Zhang H et al. 2018. Cell. 175(5):1244-1258 . PubMed
- Frank AC, et al. 2019. Nat Commun. 10:1135. PubMed
- Pan C, et al. 2019. Int J Mol Med. 44:1629. PubMed
- Dong N, et al. 2022. World J Stem Cells. 14:556. PubMed
- Wei X, et al. 2019. Int J Mol Med. 44:1425. PubMed
- Boya M, et al. 2022. Nat Commun. 13:3385. PubMed
- RRID
- AB_2566369 (BioLegend Cat. No. 368509) AB_2566370 (BioLegend Cat. No. 368510)
- Structure
- Type I transmembrane protein, 180 - 240 kD (multiple isoforms)
- Distribution
-
Hematopoietic cells, except circulating erythrocytes or platelets.
- Function
- Tyrosine phosphatases, signaling, co-stimulation (co-inhibition), TCR and BCR mediated activation.
- Ligand/Receptor
- Galectin-1, CD2, CD3, and CD4.
- Cell Type
- B cells, Dendritic cells, Neutrophils
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Protein Kinases/Phosphatase, TCRs
- Antigen References
-
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45 on UniProt.org
