Pacific Blue™ anti-human CD45 Antibody
- 产品名称：
- Pacific Blue™ anti-human CD45 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 368539
- 产品应用：
- 368539
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1488 |
1 |
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human PBMC
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC -?Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
- Application References
(PubMed link indicates BioLegend citation) -
- Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33.
- Csiba A, et al. 1984. Br. J. Cancer 50:699.
- Tchilian EZ, et al. 2001. J. Immunol. 166:1308.
- Lee MS, et al. 2004. Int. Immunol. 16:1109.
- Product Citations
-
- Frasca D, et al. 2019. PLoS One. 14:e0219545. PubMed
- Kosaric N, et al. 2020. Mol Ther. 28:2007. PubMed
- Frasca D, et al. 2021. Immun Ageing. 18:35. PubMed
- Masuda T, et al. 2022. iScience. 25:104781. PubMed
- Pellin D, et al. 2019. Nat Commun. 10:2395. PubMed
- Soto-Pantoja DR, et al. 2021. Cancer Res. 81:3890. PubMed
- Frasca D, et al. 2021. Int J Mol Sci. :22. PubMed
- Frasca D, et al. 2022. Front Aging. 2:828697. PubMed
- Frasca D, et al. 2021. Front Immunol. 616650:12. PubMed
- Frasca D, et al. 2018. PLoS One. 13:e0197472. PubMed
- Scharenberg SG, et al. 2020. Nat Commun. 2.76875. PubMed
- RRID
- AB_2716029 (BioLegend Cat. No. 368539) AB_2716030 (BioLegend Cat. No. 368540)
- Structure
- Type I transmembrane protein, 180 - 240 kD (multiple isoforms)
- Distribution
-
Hematopoietic cells, except circulating erythrocytes or platelets.
- Function
- Tyrosine phosphatases, signaling, co-stimulation (co-inhibition), TCR and BCR mediated activation.
- Ligand/Receptor
- Galectin-1, CD2, CD3, and CD4.
- Cell Type
- B cells, Dendritic cells, Neutrophils
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Protein Kinases/Phosphatase, TCRs
- Antigen References
-
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45 on UniProt.org
