Ultra-LEAF™ Purified anti-mouse/rat CD61 Ant-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2112	1

Product Details

Verified Reactivity

Mouse, Rat

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Vitronectin receptor protein from the mouse T-cell hybridoma 2B4.

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
Block, Activ, IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 104325 and 104326).

Application References (PubMed link indicates BioLegend citation)

Kieffer N, et al. 1990. Annu. Rev. Cell Biol. 6:329. (Block)
Piali L, et al. 1995. J. Cell Biol. 130:451. (Block)
Ashkar S, et al. 2000. Science 287:860. (Block)
Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block)
Moulder K, et al. 1991. J. Exp. Med. 173:343. (Activ)
Carlson TR, et al. 2008.135:2193. PubMed
Yamaji D, et al. 2009. Genes Dev. 23:2382. PubMed

RRID

AB_2832322 (BioLegend Cat. No. 104325) AB_2832323 (BioLegend Cat. No. 104326)

Antigen Details

Structure: Integrin family, 110 kD
Distribution: Platelets, megakaryocytes, endothelium, smooth muscle, activated T cells, subset of B cells, monocytes, macrophages, osteoclasts, mast cells
Function: Attachment to matrix
Ligand/Receptor: Fibrinogen, von Willebrand factor, fibronectin
Cell Type: B cells, Endothelial cells, Macrophages, Mast cells, Megakaryocytes, Monocytes, Osteoclasts, Platelets, T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Phillips DR, et al. 1991. Cell. 65:359.
3. Felding-Habermann B, et al. 1993. Curr?Opinion Cell Biol. 5:864.
Gene ID: 16416 View all products for this Gene ID 29302 View all products for this Gene ID
UniProt: View information about CD61 on UniProt.org

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